圆锥石头花中两个具有α-葡萄糖苷酶抑制活性的新三萜皂苷

摘要

目的:研究圆锥石头花根中的化学成分.方法:用75％乙醇回流提取、乙酸乙酯和正丁醇依次萃取,采用硅胶、Sephadex LH-20、Rp-C18柱色谱法和制备液相色谱等方法分离纯化,根据光谱数据(一维、二维的核磁共振谱和质谱)及酸水解鉴定了化合物的结构并进行了-葡萄糖苷酶抑制活性测定.结果:从圆锥石头花根中分离得到2个新三萜多糖皂苷--圆锥石头花皂苷A(1)和B(2),两者均具有较强的α-葡萄糖苷酶抑制活性.结论:化合物1和2是首次从该植物中分离得到的新化合物,其结构中的对甲氧基-桂皮酰基可能为决定相关活性的关键基团.%AIM:To investigate chemical constituents of Gypsophila paniculata.METHODS:The compounds were isolated by silica gel,Sephadex LH-20,Rp-C18 column chromatography and preparative liquid chromatography.Their structures were elucidated by ESI-MS,NMR techniques (1H,13C,HMQC,HMBC,TOCSY) and acid hydrolysis.Moreover their α-glucosidase inhibition activity was evaluated compared with that of their aglycone.RESULTS:Two triterpenoid saponins,caryophyllacosides A (1) and B (2),were isolated from the roots of Gypsophila paniculata,without having recourse to chemical degradation or modification.Both of them were found to have potent α-glucosidase inhibitiory activity.CONCLUSION:The two triterpenoid saponins are new boactive compounds.