113个日本血吸虫(中国大陆株)成虫表达序列标签的获取及分析

摘要

Objective To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST) .Methods A directional cDNA library constructed from Schis tosoma japonicum (Chinese strain ) adult stage RNA was used to generate expressed sequence tags(ESTs).These were compared against an EMBL-parasites database and GENBANK database by BLASTn and BLASTx. Results A total of 314 phage clones were randomly selected for generating expressed sequence tags(ESTs). From these clones, 132 EST-qu ality sequence were obtained. Among these EST-quality sequences,113 ESTs were successfully submitted to the dbEST at GenBanK. A total of 7.6% of these EST- quality sequences were previously identified sequence of Schistosoma japonicum, while 4.5% were putatively identified sequences of Schistosoma japonicum. A total of 23.5% of these EST-quality sequences were putatively identified sequence of Schistosoma mansoni or other organisms. 57.6% had no matches in the database and were classified as unknown sequences. Most ESTs with the putative prote in identified belonged to housekeeping proteins. Information about several inte resting genes was found. Conclusion Partial cDNA sequencing to generate express ed sequence tags (ESTs) has the potential to rapidly and economically increase our knowledge about adult stage Schistosoma japonicum(Chinese strain) expressed genes.%目的运用表达序列标签(Expressed Sequence Tag, EST)技术快速、节约、有效地获得有关日本血吸虫(中国大陆株)成虫表达基因的知识.方法随机挑取日本血吸虫(中国大陆株)成虫cDNA 文库单个重组克隆进行部分测序以获得EST,获得的EST通过BLAST程序同EMBL寄生虫数据库和GeneBank数据库进行比较及同源性分析.结果本研究共随机挑取日本血吸虫(中国大陆株)成虫cDNA 文库单个重组克隆314个,获得了132个有EST价值的序列,其中113个成功在GeneBank dbEST中登录.7 .6%有EST价值的序列为日本血吸虫已知序列,4.5%为日本血吸虫同源序列.曼氏血吸虫或其他生物的同源序列占有EST价值的序列的23.5%,未知序列占57.6% .通过同源性分析, 发现了几个令人感兴趣的基因,另外,大多数同源序列具有看家基因功能. 结论 EST方法具有快速、有效地获得有关日本血吸虫(中国大陆株)成虫表达基因的知识的价值.