Correlation of estrogen receptor alpha gene polymorphisms and bone mineral density in Chinese women with chronic periodontitis

摘要

＜正＞ Background Periodontitis and osteoporosis are one of the frequently encountered diseases in post-menopausalwomen.Estrogen receptors （ERs） regulated bone metabolism.To investigate the possible effect of ER-alpha （α） genepolymorphisms on bone mineral density （BMD） in pre- and post-menopausal Chinese women with chronic periodontitis（CP）,we provided sufficient quantitative information concerning the correlation between ER gene polymorphisms andBMD in periodontitis.Methods Sixty-five post-menopausal and eighty pre-menopausal CP women,and sixty post-menopausal healthyindividuals were recruited in this study.Genomic DNA was extracted from oral mucosa swab sample of each subject bythe Chelex-100 method.Determination of the ER-α polymorphisms was performed by polymerase chainreaction-restriction fragment length polymorphism （PCR-RFLP） technique with Xbal and Pvull enzyme.The index forperiodontal examination includes clinical attachment loss （CAL） and probing pocket depth （PPD）.BMD was measured bydual-energy X-ray absorptiometry （DEXA）.Results There were no significant differences between the ER-a genotypes of Pvull and Xbal and BMD inpost-menopausal and pre-menopausal CP patients,respectively （P＞0.05）.However,there was association between preandpost-menopausal CP patients at BMD of lumbar spine L2-L4 （P=0.027） and Ward’s BMD （P=0.004）.Furthermore,thepost-menopausal CP women who carried Pvull TT genotype presented significantly lower Ward’s BMD than thepre-menopausal CP women （P=0.007）,meanwhile,the post-menopausal CP women who carried Xbal AA genotypepresented significantly lower spine L2-L4 BMD than the pre-menopausal CP women （P=0.003）.Conclusions ER-α gene polymorphisms may be a susceptible indicator for BMD variation of lumbar spine L2-L4 andWard in Chinese pre- and post-menopausal women patients with CP.