目的:探讨Leica CM1950自动恒温冷冻切片机在冷冻切片中的应用.方法:根据术中送检肿物性质的不同采取相应的冷冻时间和温度,辅以不同技巧的运用,快速完成切片染色及诊断.结果:应用该切片机后术中送检组织冷冻快、冰晶少、制片简单、染色质量好,能快速准确地作出冷冻病理诊断.结论:Leica CM1950自动恒温冷冻切片机具有双压缩机、半导体制冷台,能针对不同组织设定相应冷冻温度,为术中病理诊断的准确快速提供高质量的冷冻切片.%Objective To introduce the application experience of Leica CM1950 automatic constant temperature freezing microtome in frozen section.Methods Cooling time and temperature were determined based on the natures of submitted masses,and some techniques were involved in the rapid section staining and diagnosis.Results The submitted masses after using this microtome gained advantages in freezing speed,few ice crystal,easy operation and high quality,and facilitated accurate pathologic diagnosis.Conclusion Leica CM1950 automatic constant temperature freezing microtome has double compressor and semiconductor refrigeration units,can set corresponding freezing temperature for different organizations and provide high-quality frozen section for accurate and rapid intraoperative pathological diagnosis.
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