一种用于战创伤急救护理的“液体绷带”研制

摘要

Objective To develop a liquid bandage for self aid of war wound.Methods Solution A and B were prepared containing functional polymers derived from the biocompatible poly (ethylene glycol) and hyaluronic acid,which had ability of fast crosslinking reaction when they were mixed.Among them,A solution mainly comprised of mercapto group-containing hyaluronic acid derivative (HA-SH),water-solubility starch,propylene glycol,benzoic acid and etc.B solution contained pentaerythritol tetraacrylate derivative (4arm-PEG-Acrylate) and benzoic acid.The structure of mercapto group-containing hyaluronic acid derivative was confirmed by IR,1H-NMR and etc.HA-SH and 4arm-PEG-Acrylate were formulated to different concentrations (W/V) in different buffers.The two kinds of solution were mixed in different ratios,and in situ crosslinking hyaluronic acid hydrogel was obtained;the crosslinking time was recorded.The adhesive force and waterholding capacity of the hydrogel after crosslinking were measured by adding excipients such as water-solubility starch.Results In case the concentration of HA-SH and 4arm-PEG-Acrylate was 2％ and pH value was 11,the hydrogel film on wound skin would be quickly formed in 20 seconds for wound dressing after spraying A and B solution onto the wound skin.Conclusion The liquid bandage has strong adhesive force,high water-holding capacity and controllable crosslinking time,so it could be used as a novel wound dressing for war wound.%目的:开发一种“液体绷带”,用于战创伤的紧急自救护理.方法:以生物相容性高分子聚乙二醇和透明质酸的衍生物为主要骨架,通过对其功能化改性得到2种可快速交联的聚合物,将这2种聚合物分别制备成A和B2种反应溶液:A溶液由巯基质酸衍生物(HA-SH)、水溶性淀粉、丙二醇、苯甲酸等组成;B溶液由四甲基丙烯酸酯(4arm-PEG-Acrylate)衍生物、苯甲酸等组成.通过傅里叶变换红外光谱仪、核磁共振氢谱(1H-NMR)对巯基质酸衍生物结构进行表征.采用不同缓冲液将HA-SH和4arm-PEG-Acrylate配制成一定浓度(W/V)溶液,按照不同比例混合,获得交联透明质酸水凝胶,记录交联反应时间.通过加入水溶性淀粉等辅料,测定交联后水凝胶的黏附性和保湿性.结果:HA-SH和4arm-PEG-Acrylate的浓度为2％、pH值为11时,将A和B溶液分别喷涂于患者伤口处,在20 s内快速发生交联形成水凝胶敷贴,凝胶成型性能好.结论:“液体绷带”成型时间短,有较好的黏附性和保湿性,有望成为一种新型的伤口敷料.