目的 研究血吸虫重组BCG-Sj26GST疫苗对小鼠T淋巴细胞亚群的影响。方法 实验一采用106和108CHU疫苗皮下接种BALB/c小鼠，接种后8WK用日本血吸虫尾蚴进行攻击感染，感染6wk剖杀小鼠，取脾制备脾细胞，同时设有PBS对照组。实验二分别用106CHJ疫苗皮下和静脉注射免疫小鼠，分别于免疫后0、4、8、10、14和16wk各剖杀4只，分离脾脏，用FACsort流式细胞仪分析T细胞CD+4和CD+8亚群百分比。结果 实验一疫苗免疫组小鼠经尾蚴攻击后脾T细胞CD+4亚群明显升高，CD8+亚群缓慢增加。实验二动态观察发现在皮下和静脉注射组，CD+4亚群分别于免疫后10和18wWK显著增加；皮下注射组CD8+亚群在整个观察期间均无明显变化，静脉注射组则于免疫后4～16wk有轻度增加。结论 T细胞CD+4亚群可能在疫苗保护性免疫中起重要作用。%Aim To study the effects on T lymphocyte subsets in spleen of mice immunized with recombinant BCGSj26GST. Methods Inthe first experiment, BALB/c mice were immrnized subcutaneously by 106 and 108 CFU BCG-Sj26GST respectively. ALLthe mice were artifically challenged with cerariae of Schistosoma japonicum on 8weeks after immunization, six after challenge, the mice were killed, and the spleens were removed, cells were prepared seperately and were analysed by immunofluorescence with conjugated monoclonal antibodies in a FACsort cytofiuorimeter at 523 nm, PBS treated mice were serwed as control. In the second experiment, after immunized subcutanenously or inntraveously by 106 CFU vaccine, 4 mice were ranndonly killed to separate spleens on 0wk, 4wk, 8wk, 10wk, 14wk and 16wk after immurization, the percentage of CD+4 and CDs+ subsets was analysed as above. Results In the first experiment, CD+4 subsets increased renarkably, but CD+8 subsetsdid slightly by immunization with the vaccine against challenge with S.j. Cercariae, in the second experiment, the dynamic observation showed that in the subcutaneous and intravenous group, CD+4 subsets increased obviously since 10wk and 8wk respectively, CD8+ subsets hadno obvious changeover 16 weeks of observation , in the subcutaneous groupbuttheCD8+ subsets rose lightly on 4-16wk in the intravenous group. Conclusion On the basis of this study it's suspected that CD+4 subsets might play an important role in the protective immunity induced by the vaccine
展开▼
