The objective was to survey the epidemic locus of paragonimiasis in Shennongjia and provide the basis for drawing up adequate control strategy . The serum antibody of the people in the area was detected by DIGFA-kit and compared with the result by ELISA . The infection situation of intermediate host and reservoir host in the area was investigated simultane -ously . A total of 1 128 serum samples collected from the population , in which 76 were positive with a rate of 6 .74% .The masculine coincidence rate was 98 .7% by ELISA . The infection rate of snail which considered as the first intermediate host was 0 .91% , the infectiosity of limnetic crab which considered as the second intermediate host was 3 .21 larvae per crab , and the natural infection rate of cat and civet cat was 51 .72% and 15 .79% , respectively . The existence of the first and the second intermediate hosts and the reservoir hosts suggests that this area is a natural focus of paragonimiasis skrjabini .%目的 探讨神农架林区并殖吸虫病疫源地的分布情况,为预防控制并殖吸虫病提供科学依据.方法 应用 DIGFA-kit检测神农架林区人群血清抗体,并与常规ELISA相比较;同时调查中间宿主、动物宿主感染情况.结果 共采集人群血清样本1 128份,阳性76份,阳性率为6.74%,ELISA平行对照试验阳性符合率为98.7%.第一中间宿主螺的感染率为0.91%,第二中间宿主淡水蟹感染囊蚴为3.21个/只,动物宿主猫和果子狸的自然感染率为51.72%和15.79%.结论 DIGFA-kit具有较好的灵敏度和特异性,适合于流行区临床检验和大规模流行病学调查.调查区域有第一、第二中间宿主和保虫宿主的存在,为斯氏狸殖吸虫病自然疫源地.
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