沙鼠肝泡球蚴组织中骨桥蛋白与IL-10的表达及相关性研究

摘要

目的 检测沙鼠肝泡状棘球蚴组织中骨桥蛋白(OPN)及白细胞介素-10(IL-10)的表达,并进一步探讨两者的相关性.方法 20%泡状棘球蚴组织混悬液开腹直视下肝脏穿刺注射感染长爪沙鼠60只,每只0.1 mL,建立肝泡状棘球蚴长爪沙鼠模型.随机分为模型组(无干预,40只)和实验组(抗OPN抗体干预,20只).感染至100 d时剖杀所有长爪沙鼠,观察泡状棘球蚴生长和转移情况,取长爪沙鼠肝泡状棘球蚴组织和转移灶标本.采用免疫组织化学SP法观察沙鼠肝泡状棘球蚴组织和转移灶中OPN及IL-10的表达情况.结果 60只长爪沙鼠全部感染肝泡状棘球蚴.免疫组化染色显示肝泡状棘球蚴组织可见OPN及IL-10不同程度的表达.模型组中OPN和IL-10阳性细胞表达率分别为72.5%(29/40)和65%(26/40),OPN及IL-10主要分布在肝泡状棘球蚴纤维囊壁,阳性产物均定位于细胞浆.65%(26/40)的长爪沙鼠模型经病理证实发生胸廓内淋巴结转移,伴有胸廓淋巴结转移的肝泡状棘球蚴组织中OPN及IL-10阳性细胞表达率分别为84.6% (22/26)、76.9%(20/26),均高于未发生胸廓淋巴结转移的肝泡状棘球蚴组织(50%、42.9%)(P<0.05).实验组OPN和IL-10的阳性细胞表达率分别为40%(8/20)和35%(7/20).OPN与IL-10表达呈正相关(r=0.605,P=0.000).结论 OPN阳性表达可能与肝泡状棘球蚴的转移有关,OPN与IL-10可能在肝泡状棘球蚴的生长中起协同作用.%To investigate the expressions of osteopontin and interleukin-10 and their correlation in Gerbil hepatic alveolar hydatid tissue, 60 gerbils were infected with 20% E. Mullilocularis suspension (0. 1 mL per gerbil) through abdominal opening injection in liver. They were randomly divided into model group (No intervention, 40 gerbils) and experimental group (Anti-OPN antibody interference, 20 gerbils). Gerbils were sacrificed at 100 days post-infection for observing the growth and metastasis of hepatic echinococcus cyst. The expressions of OPN and IL-10 in hepatic echinococcus cyst and metastasis tissue were measured by immunohistochemistry staining (SP method). Results showed that all gerbils infected with hepatic alveolar hydatid. Expression of OPN and IL-10 were found at different degree in echinococcus cysts. In model group, the positive expression rate of OPN and IL-10 in echinococcus cysts were 72. 5% (29/40) and 65% (26/40). Both OPN and IL-10 were mainly distributed in the germinal layer. The 65% (26/40) of them occurred thoracic lymph node metastases. The OPN and IL-10 expression rate in hepatic echinococcus cysts with thoracic lymph node metastases were 84. 6% (22/26) and 76. 9% (20/26) , which were significantly higher than that of hepatic echinococcus cysts without thoracic lymph node metastases (50% , 42. 9%) (P<0. 05). In experimental group, the positive expression rates of OPN and IL-10 in echinococcus cysts were 40% (8/20) and 35% (7/20). The expression of OPN was significantly correlated with the expression of IL-10 (r=0. 605 , P=0. 000). Inconclusion, OPN might significantly relate to the metastasis of hepatic echinococcus cysts. OPN and IL-10 might play synergetic roles in hepatic alveolar hydatid tissue.