口蹄疫病毒致乳鼠心肌炎的动物模型

摘要

Interactions between FMDV and cardiac cells are multifaceted and complex ,these interactions leads to pro-teins alterations in cardiac cells inevitably .To understand the pathogenesis of myocarditis after FMDV infection in mice ,the suckling mouse model for myocarditis induced by foot-and-mouth disease virus (FMDV) was established in this study .Suckling mice within 3 days old was selected to infect by FMDV .Myocarditis caused by FMDV in suckling mice was confirmed with clinical symptom monitor .The observation of Hematoxylin and eosin stain (H&E stain) and transmission electron microscopy (TEM) were performed after samples processing .According to conventional polymerase chain reaction (PCR) methods ,prim-ers of VP1 gene was designed ,synthesised and specific FMDV VP1 gene was amplified from the heart muscle of suckling mice . The results indicated that suckling mice appeared low spirit condition ,dyspnea ,and dull reaction within 36 hours after chal-lenge with FMDV .Infiltration of inflammatory cells and dissolution of myocardial fibers were observed with H&E stain and TEM .Special target gene of FMDV was amplified from the heart of infected group .Obvious inflammation in the heart of suck-ling mice caused by FMDV was observed .It's suggested that suckling mouse model for myocarditis induced by FMDV was es-tablished successfully ,which would lay the foundation for researches of myocarditis mechanism in young cloven-hoofed ani-mals .%目的：为建立乳鼠感染FMDV心肌炎的动物模型。方法本研究以3日龄乳鼠为研究对象，经病毒接种，饲养观察，乳鼠心肌 H ．E染色，乳鼠心肌透射电镜显微观察和乳鼠心肌FMDV特异性目的基因扩增。结果结果显示接种FM-D V后36 h内乳鼠频临死亡，H ．E染色和透射电镜观察可见乳鼠心肌明显炎症细胞浸润，心肌纤维部分溶解，感染组乳鼠心肌中检测到FMDV特有的VP1基因。结论建立了乳鼠感染FMDV心肌炎的动物模型，为研究FMDV致幼龄偶蹄动物心肌炎的分子机制建立了动物模型。