48株鲍曼不动杆菌氨基糖苷类抗生素耐药基因检测

摘要

To investigate the distribution of the resistance genes of Acinetobacter baumannii to aminoglycoside,48 strains of extensively drug resistant Acinetobacter baumannii were collected from the First Affiliated Hospital of Bengbu Medical College from January to December,2015.The drug sensitivity test and identification were performed by VITEK 2 compact automatic microorganism instrument.Twelve aminoglycosides modifying enzymes,three 16SrRNA methylase genes and efflux pump abeB gene were detected from these isolates by PCR.Results showed that among these experimental 16 genes,aac(6')-Ⅰb gene was detected from 19 of 48 isolates (39.6％),both armA and adeB genes were 43 (89.6％),ant(3")-Ⅰa gene was from 5 (10.4％),while the other genes were not found.And more than two gene types were amplified from 39 of 48 strains (81.3％).In conclusion,the aac(6')-Ⅰb,armA gene and efflux pump adeB may play a key role in drug resistance to aminoglycosides antibiot ics of Acinetobacter Baumanni in our hospital.%目的 了解鲍曼不动杆菌氨基糖苷类抗生素耐药基因分布情况.方法 收集蚌埠医学院第一附属医院2015年1月至12月临床分离的48株泛耐药鲍曼不动杆菌,VITEK 2 Compact进行鉴定和药敏实验.PCR法检测12个氨基糖苷类修饰酶基因和3个甲基化酶基因以及外排泵基因adeB.结果 在实验的16个基因中,共检出4种氨基糖苷类抗生素耐药基因aac(6')-Ⅰb、armA、adeB和ant(3”)-Ⅰa,其中aac(6')-Ⅰb检出率为39.6％(19/48),armA基因检出率为89.6％(43/48),adeB检出率89.6％(43/48),ant(3")-Ⅰa检出率为10.4％(5/48),其余基因均未检出;存在两种以上耐药基因的共39株,检出率为81.3％(38/48).结论 aac(6') Ⅰb、armA基因以及外排泵adeB是介导我院鲍曼不动杆菌氨基糖苷类抗生素耐药的主要基因.