目的 研究人工建立肝脏接种泡球蚴模型后小鼠的死亡规律及主要死亡原因,探讨造模术后导致死亡的主要并发症的风险因素及预防.方法 开腹直视肝脏穿刺法接种50只健康昆明小鼠,术后15 d内动态观察小鼠的生理变化并记录死亡时间与数量,着重分析其死亡原因及相关因素.结果 术后15 d共死亡19只,第9 d死亡1只,其余18只在术后7 d内死亡.术后死亡量于第2d开始上升,第4、5d达高峰后逐渐下降至第7d死亡1只.结论 泡球蚴接种术后小鼠的主要死亡原因与手术方式、麻醉、感染、泡球蚴的抗原性、肝损伤等导致的并发症密切相关.有效预防此类并发症是提高造模生存率的关键.%We studied the death pattern and the main causes of death after artificial liver inoculation with alveolar hydatid model,and explored the risk factors and prevention of major complications resulting from death after modeling.The 50 healthy Kunming mice were inoculated with open-staring liver puncture and the physiological changes of the mice were observed dy-namically within 1 5 days after operation.The time and amount of death were recorded,and the causes of death and related fac-tors were analyzed emphatically.On the 1 5 day after surgery,1 9 died;1 died in the ninth day,and the remaining 1 8 died with-in 7 days of the operation.The postoperative death rate began to rise in the second day,and gradually decreased to 1 after the peak of the 4 th and 5 th days.The main causes of death of the mice after inoculation were closely related to the complications of surgical methods,anaesthesia,infection,antigenicity of vesicle,and liver injury.Effective prevention of these complications is the key to improve the survival rate.
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