近红外光谱法快速检测兽用冻干疫苗中的水分含量

摘要

The article developed determination method of water content in animal vaccines with integrate sphere diffuse reflectance NIR spectrometer, and established quantitative calibration models with PLS algorithm and full cross - validation, SECV was 0. 0293, SEP was 0. 0493 and R^2 was 0. 9936. The predicted deviation of 27 samples distributed within the range of - 0.47 - 0.63, RSD was 0.19% - 2.24%, F test indicated that NIR method and Karl Fischer method showed no significant difference in water detection of animal vaccines. The experiment provided important reference value in animal vaccines water detection with NIR method.%利用积分球漫反射近红外光谱法检测了7种动物用冻干疫苗中的水分含量。采用PLS算法，全交互验证建立定量校正模型，SECV为0．0293，SEP为0．0493，R^2为0．9936。对27个样本的预测偏差范围为-0．47—0．63，相对标准偏差为0．19％～2．24％，F检验表明近红外光谱法检测动物疫苗中水分含量与卡尔费休法无明显差异，为近红外方法在动物疫苗水分含量无损检测的应用提供了依据。