贵州省部分地区H7亚型禽流感监测与分析

摘要

为了解贵州省H7亚型禽流感感染情况,采用HI试验对全省9个地区家禽血清样本进行血清抗体检测,PT-PCR方法对家禽咽-肛拭子、养禽场环境样本和野鸟粪便进行病原核酸检测.结果显示:在1570份家禽血清样本中,H7亚型禽流感血清抗体阳性率为0.45％(7/1570);在350份家禽咽-肛拭子和310份养殖场环境样本中,均未检测出H7亚型禽流感病毒核酸;在160份野鸟粪便中,有1份为H7亚型禽流感病毒核酸阳性,阳性率为0.63％(1/160).试验提示,在贵州省养禽场中不存在H7亚型禽流感感染,但迁徙野禽中可能存在H7亚型禽流感隐性感染,应当引起重视.%To master the infection situation of H7 subtype avian influenza (AI-H7) in Guizhou province,the serum samples of poultry were collected to detect the AI-H7 antibodies by HI test,and the samples of swallow-anal swabs and environment and wild bird faeces were gathered to determine the pathogenic nucleic acid of AI-H7 by RT-PCR method.The results showed that the positive rate of AI-H7 antibodies was 0.45％ in 1570 of poultry serum samples.And the pathogenic nucleic acid of AI-H7 were not detected in 350 of swallow-anal swabs and 310 of farm environment samples,but 1 sample in 160 of wild bird faeces which the positive rate was 0.63％.These results suggested that there were no infection of AI-H7 in poultry farm,but maybe latent infection in wild birds and should be paid attention.