H5 H7 Real-time PCR Diagnostic Kit for Simultaneous Detection of H5 and H7 Subtype Avian Influenza Virus

摘要

The M/H5/H7 type AI real-time genetic diagnosis method currently used for AI national surveillance and diagnosis is an internationally representative and generally used method using standard primers and probes made based on the avian influenza virus prevalent in Europe. , Due to the rapid mutation of AI virus recently, the need to develop a diagnostic kit optimized for epidemic strains in Eurasia where there is a high possibility of domestic introduction has been raised. The present invention relates to a diagnostic kit that newly selected Primer and Probe optimized through analysis of domestic isolates and Eurasian epidemic strains and applied real-time genetic diagnostics using them. As a result of experiments on various domestic isolates and field clinical samples with the diagnostic kit of the present invention, it was confirmed that it showed high sensitivity compared to the current method, and it was also confirmed that the specificity was high. By applying the present invention to AI national surveillance and diagnosis, it is expected to minimize damage to the poultry industry and contribute to national quarantine by rapidly and accurately diagnosing H5/H7 type highly pathogenic avian influenza as well as avian influenza virus, resulting in large social and economic expectations. do.