超声微泡击破技术增效激光消融兔肝VX2肿瘤的实验研究

摘要

Objective To investigate the basis of ultrasound microbubble technology enhanced thermal ablation effect.Methods All 48 rabbits bearing hepatic tumors were averagely randomly divided into four groups:only laser group,sham,destruction + laser group,and blank group.The filling defect area changes of before and after treatment were observed and the differences between groups were compared.Results Pathology confirmed ablation of tumor tissue could produce coagulation necrosis for experiment group.The conventional ultrasound showed there was no significant variation in the maximum diameter and area in the pre and post treatment of experiment group and control group(P ＞0.05).There was no change for the control group.Ultrasound imaging showed an area of filling defect were:laser treatment alone group (0.83 ± 0.06) cm2,microbubbles false break combined with laser therapy group (0.65 ± 0.05) cm2,microbubbles break combined with laser therapy group (2.40 ± 0.16)cm2,blank control group (0.01 ± 0.01) cm2.The filling defect area of the treatment group,which was combined with ultrasound microbubble break technology and laser ablation was increased,there was significant variation in the each group (P ＜0.05).Conclusions Microbubble burst combined laser ablation could expand the single needle laser ablation area of rabbit VX2 liver tumor and increase the therapeutic effect.%目的 探讨超声微泡击破技术增强激光热消融的治疗效果.方法 将48只荷瘤兔随机分为单纯激光治疗组、假微泡击破+激光治疗组、微泡击破+激光治疗组、空白对照组,通过影像学、解剖学、病理学指标观察治疗前后的形态学变化,比较各组间的差异.结果 病理证实实验组激光热消融治疗对肿瘤组织均产生了凝固性坏死,常规超声显示实验组治疗前后及对照组肿瘤最大直径和面积差异均无统计学意义(P＞0.05),对照组无治疗后的改变.超声造影显示消融区造影剂充盈缺损的面积分别为:单纯激光治疗组(0.83±0.06)cm2,假微泡击破+激光治疗组(0.65±0.05) cm2,激光+微泡击破治疗组(2.40±0.16)cm2,空白对照组(0.01±0.01) cm2.微泡击破技术联合激光消融治疗肿瘤所产生的造影剂充盈缺损的面积各组之间差异有统计学意义(P＜0.05).结论 微泡击破联合激光消融明显扩大单针激光消融兔肝VX2肿瘤的面积,可增加治疗的效果.