单核细胞趋化蛋白-1基因多态性与肺结核易感性的关系

摘要

目的 探讨中国汉族人群单核细胞趋化蛋白-1(MCP-1)基因-2518位点多态性与肺结核发病的关系.方法 2008年3-8月采用1:1配对病例对照设计,用PCR-限制性片段长度多态性分析(RLFP)检测167例肺结核患者和167例对照的MCP-1基因-2518位点的基因型分布.病例组年龄16～77岁,对照组年龄15～78岁,两组患者的中位年龄均为43岁,四分位间距均为30岁;两组均为男110例、女57例,汉族.对一般情况、现病史、既往病史及肺结核相关环境因素进行问卷调查,并进行单因素和多因素条件logistic回归分析.结果 AA、GA和GG基因型在病例组的分布频率分别为21/167(12.6%)、62/167(37.1%)和84/167(50.3%),在对照组的分布频率分别为42/167(25.2%)、83/167(49.7%)和42/167(25.1%).单因素分析结果显示,基因型在病例组和对照组中分布频率的差异有统计学意义(x2=24.041,P<0.01);调整环境因素后,多因素回归分析结果显示,GG基因型与肺结核发病仍有显著性相关性(OR=1.989,95%CI为1.154～3.428,x2=6.124,P<0.05).结论 MCP-1基因-2518位点GG基因型可能与中国汉族人群的肺结核易感性有关.%Objective To explore the possible relationship between the -2518 A/G single nucleotide polymorphisms in monocyte chemoattactant protein-1 ( MCP-1 ) gene of Chinese Han population and the susceptibility to pulmonary tuberculosis. Methods A 1:1 matched case-control study was conducted from March 2008 to August 2008. The - 2518 MCP-1 A/G polymorphisms were detected in 167 pairs of subjects by reaction-restriction fragment length polymorphism (PCR-RLFP) technique. General characteristics, current disease history, past medical history and related environmental factors of tuberculosis were collected using a questionnaire designed by ourselves. Univariate analyses and multivariate conditional logistic analyses were conducted. Results The genotype frequencies of AA, GA and GG in the case group and the control group were 21/167 (12.6% ), 62/167 (37.1% ), 84/167 (50.3% ) and 42/167 (25.2%), 83/167 (49.7% ), 42/167 (25.1%), respectively. There was a significant difference in genotype frequencies between the case and the control groups in the univariate analysis ( x2=24.041, P < 0.01). The significant association remained after adjusting environmental factors between genotype GG (OR 1.989, 95% CI 1.154 -3.428,x2= 6.124, P <0.05) and the susceptibility to pulmonary tuberculosis. Conclusion The -2518 MCP-1 GG genntype might be associated with susceptibility to pulmonary tuberculosis in Chinese Han population.