活化T细胞核因子和单核细胞趋化蛋白-1及该蛋白2518G/A基因多态性与冠心病的关系

摘要

Objective: To investigate the relationship between nuclear factor of activated T cells( NFAT ), monocyte chemoattractant pro-tein-1 ( MCP-1 ) with MCP-1-2518G/A gene polymorphism and the severity of coronary artery disease ( CAD ).rnMethods:According to coronary angiography ( CAG ) examination, the patients were divided as Acute coronary syndrome ( ACS) group,n= 180,Stable angina pectoris( SAP) group,n = 120 and Control group,n = 60,the patients had normal CAG. The severity of coronary lesions was analyzed by Gensini coronary score system,the protein concentrations of NFAT and MCP-1 were measured by ELISA,and MCP-1-2518 G/A gene polymorphism was examined with PCR-RFLP.rnResults: ①he protein concentrations of NFAT and MCP-1 were both higher in ACS group than those in SAP group and Control group,P<0. 05 respectively. Plasma level of MCP-1 was positively related to NFAT ( r = 0. 65 ,P<0. 05 ). ②Gensini score of coronary lesions was positively related to NFAT, MCP-1, LDL-cholesterol and blood sugar, while negatively related to HDL-cholesterol. ③There were 3 polymorphisms in MCP-1-2518G/A gene,GG type had higher MCP-1 than AG type and AA type, P<0. 05 respectively. In ACS group, GG type and G Allen frequency were both higher than those in SAP group and Control group, P<0. 05 respectively.rnConclusion:The elevated plasma levels of NFAT,MCP-1 and MCP-1 gene polymorphism are related to the severity of coronary lesions in CAD patients.%目的:研究活化T细胞核因子和单核细胞趋化蛋白-1及该蛋白2518G/A基因多态性与冠心病病变程度的相关性.方法:选取冠心病患者300例,根据冠状动脉(冠脉)造影结果确诊为冠心病的患者分为急性冠脉综合征(ACS)组180例,稳定型心绞痛组120例.另选冠脉造影正常者为对照组60例.应用Gensini评分评定狭窄程度,酶联免疫法测定活化T细胞核因子(NFAT)、单核细胞趋化蛋白-1(MCP-1)浓度,多聚酶链反应--末端限制性片段长度多样性(PCR-RFLP)方法检测MCP-1 2518G/A基因多态性.结果:①急性冠脉综合征组的NFAT、MCP-1浓度显著高于稳定型心绞痛组及对照组,差异有统计学意义(P<0.05),MCP-1浓度与NFAT呈正相关(r=0.65,P<0.05).②冠脉病变Gensini评分与NFAT、MCP-1、低密度脂蛋白胆固醇、血糖呈正相关,与高密度脂蛋白胆固醇呈负相关.③MCP-1 2518G/A基因有三个基因型,GG型的MCP-1浓度较AG型及AA型升高(P<0.05),ACS组GG型基因和G等位基因频率较稳定型心绞痛组及对照组增高,差异均有统计学意义(P<0.05).结论:冠心病患者血浆NFAT与MCP-1水平的升高及MCP-1 2518G/A基因多态性与冠脉病变程度相关.