香蕉棒孢霉叶斑病菌的分离与鉴定

摘要

Three isolates were obtained from banana leaf spot sanples collected from Nanning, Guangxi by tissue isolation.The pathogenicity of the isolates was confirmed based on Koch's postulates.The conidia and conidiophores produced on the PDA-incubated colonies became longer than those on naturally occurred banana leaf spots.The pathogenic isolate CATAS100 was identified as a member of Corynespora cassicola based on its morphological characters on PDA medium and rDNA-ITS (ribosomal DNA internal transcribed spacer) sequence.This is the first report on molecular identification of Corynespora cassiicola causing banana leaf spots in China.%从广西南宁市采集香蕉棒孢霉叶斑病样本,用常规组织分离法获得3个菌株,经柯赫氏法则验证确定其为香蕉叶斑病的病原菌.该菌的形态特征在分离培养前后变化较大,自然病斑上的分生孢子和分生孢子梗均较短,经PDA培养基分离培养后显著变长,培养后的病原菌彤态特征与前人对Corynespora cassiicola的描述一致.根据形态特征及rDNA-ITS区序列比对结果,确定该病原菌株为多主棒孢菌[Corynespora cassiicola(Berk &Curt)Wei].