农杆菌介导蔗糖:蔗糖果糖基转移酶基因转化木薯的研究

摘要

Sucrose:sucrose 1 -fructosyltransferase (1 -SST)is one of the key enzymes which mainly involves in biosynthesis of fructan, the resistance of plant in a variety of environmental stress is enhanced with fructanaccumulation. In this study, 1 -SST gene was successful introduced into cassava SC6 genome by Agrobacterium-mediated transformation. The results showed that, the best screening concentration of PPT was 2.0 mg/L while budding, 71 resistant plants were obtained, two positives among them were found by PCR and RT-PCR analysis. Southern blot analysis showed that 1-SST gene was integrated into the genome of one transgenic cassava plant.%蔗糖:蔗糖果糖基转移酶1-SST是果聚糖合成代谢过程的关键酶,而果聚糖的积累可以增强植物在多种环境胁迫下的抗逆性.通过农杆菌介导法将1-SST基因导入木薯品种SC6中.结果表明:芽器官发生时PPT的最佳筛选浓度为2.0 mg/L,共得到71株抗性再生植株.对抗性植株进行了PCR、RT-PCR鉴定,其中阳性植株2株,经Southern杂交分析,最终获得1株转基因木薯植株.