漳州水仙随体染色体的分离及DNA文库的构建

摘要

以漳州水仙为材料,采用微细玻璃针分离了水仙第7号同源组的3条随体染色体,进行了LA-PCR体外扩增,Southern杂交证明水仙单染色体DNA被成功扩增.将扩增产物插入PMD18-T载体构建了5.0×105个克隆的单染色体DNA文库,为进一步研究中国水仙的起源与进化提供了有益的细胞遗传学和分子生物学依据.%In order to explore the origin of chromosomes of Zhangzhou Narcissus, 3 satellite chromosome wasrnisolated by glass needles, and the chromosomal DNAs were amplified by LA-PCR. Southern blot revealed that thernPCR products were homogeneous with the Zhangzhou Narcissus genomic DNA, indicating that DNAs from thernsatellite chromosome had been successfully amplified. The PCR products were cloned into PMD18-T vector andrnabout 5.0xl05 recombinant clones were obtained.