参威骨痹片对骨关节炎模型兔膝关节液IL-1β、 TNF-α的影响

摘要

Objective:To observe the effects of Shenwei Gubi Tablet on interleukin 1 beta (1L-1β) and tumor necrosis factor alpha (TNF-α) in synovial fluid of knee osteoarthritis model rabbits.Methods:40 healthy New Zealand rabbits were randomly divided into 4 groups:normal group,model group,Chinese medicine (Shenwei Gubi Tablet) group and positive group.The knee osteoarthritis (OA) models were reproduced by the Hulth method.After modeling successfully 4 weeks,the positive group was gavaged with diacerein 5.45 mg/kg,Chinese medicine group with Shenwei Gubi Tablet 10.464 g/kg for 4 weeks,then normal feeding 1 week,IL-1βand TNF-αcontents in rabbits' synovial fluid were determined by ELISA method.Results:The synovial fluid IL-1 βand TNF-αcontents in model group rabbits were higher than those of normal group(P ＜ 0.05),synovial fluid TNF-αcontent in Shenwei Gubi Tablet group and positive group were lower than that in model(P ＜ 0.05),synovial fluid IL-1 βin positive group was lowered significantly (P ＜ 0.05).Conclusion:Shenwei Gubi Tablet and decrease synovial fluid TNF-α content of knee osteoarthritis model rabbits,inhibit inflammatory reaction,which is one of the mechanism of Shenwei Gubi Tablet effectively treating KOA.%目的:观察参威骨痹片对骨关节炎模型兔膝关节液内白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)水平的影响.方法:选择40只健康新西兰兔,随机分为4组:分别为正常组、模型组、中药组、阳性药物组.用Hulth方法复制兔膝骨关节炎模型.术后4周,阳性药物组用双醋瑞因(5.45 mg/kg)灌胃、中药组用参威骨痹片(10.464 g/kg)灌胃,给药4周.正常饲养1周后,ELISA测定各组兔关节液IL-1β、TNF-α含量.结果:与正常组比较,模型组兔关节液IL-1β、TNF-α含量升高(P＜0.05);与模型组比较,阳性药物组和参威骨痹片组兔关节液TNF-α含量下降(P＜0.05),但仍高于正常组(P＜0.05);阳性药物组IL-1β含量低于模型组(P＜0.05),参威骨痹片组IL-1β含量有所降低,但数据处理无统计学意义.结论:参威骨痹片能够降低膝骨关节炎兔关节液TNF-α含量,抑制炎症反应,是其治疗KOA的作用机制之一.