目的由于 Pax-2对肾细胞癌具有较高特异性,可以作为肾细胞癌的一种肿瘤标志物,因此探讨 Pax-2在肾细胞癌患者外周血中的表达及其对微转移的意义. 方法用 RT-PCR法检测 25例患者外周血中的 Pax-2 mRNA表达,以 10例其他肿瘤患者和 5例健康人为阴性对照,以β-actin为内对照. 结果 19例患者外周血中有 Pax-2 mRNA的表达(阳性率为 76%),其他肿瘤和正常人中有 1例表达 Pax-2 mRNA(阳性率为 6.7%),差异有显著性(χ 2=18.027,P< 0.01). Pax-2 mRNA的表达与肿瘤分类、临床分期无相关性(χ 2=3.206,3.181,P >0.05). 结论外周血中 Pax-2 mRNA的检测可以较敏感地检测到血液中肾癌细胞 ,有助于早期诊断肾细胞癌及其微转移,从而改善患者的预后,提高生活质量.%Aim Pax-2 has a higher specificity to renal cell carcinoma and can be as a tumor maker of renal cell carcinoma.This article explored the expression of Pax-2 in peripheral blood of renal cell carcinoma patients and its significance in controlling micrometastasis.Methods The expressions of Pax-2 in peripheral blood of 25 patients with renal cell carcinoma were detected with reverse transcription PCR(RT-PCR).10 patients with other tumor and 5 healthy people were as the negative control and β-actin was as internal control.Results Among 25 peripheral blood specimens,19 were positive(with positive rate of 76% ).There was only one positive case in the control group with positive rate of 6.7% .There was significant difference between two groups(χ 2=18.027,P< 0.01) .But there were no significant correlation between Pax-2 mRNA expression and tumor classification and clinical staging(χ 2=3.206,3.181,P >0.05) .Conclusion The detection of Pax-2 mRNA is sensitive for tumor cells in peripheral blood,which benefit early diagnosis of renal cell carcinoma and its micrometastatsis,then the prognosis of patients can be turned better and quality of life is improved.
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