Aim Numerous studies using experimental animals have also confirmed the important role of hippocampus somatostatinergic systems in cognitive processes.The purpose of this study was to see if a significant loss of hippcampus somatostatin - containing neurons of neurons after experiment diffuse axonal injury (DBI).Methods The present study adapted the model which Marmarou et al,developed a model of brain impact - acceleration injury were examined by adjusting the height of the falling weight to either 1 m(mild injury group) or 2 m(severe injury group).Morphological studies were performed in serial sections,using immunohistochemical SP staining method of somatostatin - containing neurons.Results The number of somatostatin - containing neurons in hippocamus were apparently decreased as compared with control group(P< 0.01).The number of neurons in severe injury group was aparently decreassed as compared with mild injury group(F=21.789-125.205,P< 0.01).The loss of neurons at two weeks was apparently different from those at one week after insult(t=1.833-6.038,P< 0.01 or P< 0.05).Conclusion A singnificant and apparently selective loss of hippocamus somatostatin - containing neurons following DAI is demonstrated in this study.This type of injury may be one of important pathological changes of cognitive dysfunction after diffuse axonal injury.Meanwhile,second injury plays a very important role in the loss of this type neurons.%目的弥漫性轴索损伤( DAI)能导致伤后认知障碍.通过建立实验性 DAI动物模型,了解在 DAI伤后与认知功能关系密切海马生长抑素( Ss)样神经元的变化.方法采用 Marmarou打击装置建立 DAI动物模型,免疫组织化学染色以显示海马 Ss 样神经元.结果海马 Ss 样神经元在重伤组、轻伤组、对照组差异有非常显著性意义( F=21.789～ 125.205,P< 0.01);损伤后 2周组神经元减少与 1周组比较差异有显著性意义( t=1.833～ 6.038,P< 0.01, 0.05).结论 DAI后海马 Ss 样神经元的减少可能是伤后认知障碍,甚至是 DAI后植物生存的主要病理改变之一.伤后的迟发性细胞死亡在该种神经元的减少中起着重要作用.
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