低频脉冲超声干预膝骨关节炎模型兔关节软骨及滑膜水通道蛋白3的表达

摘要

BACKGROUND: Previous studies demonstrated that, low-frequency pulsed ultrasound can promote repair of full-thickness articular cartilage defects, delay the progression of knee osteoarthritis. Aquaporin 3 exists on articular cartilage and synovial membrane, which plays an important role in osteoarthritis.OBJECTIVE: To observe the effect of low-frequency pulsed ultrasound on the expression of articular cartilage and synovial aquaporin 3 in rabbits with knee osteoarthritis.METHODS: Experimental osteoarthritis was replicated in knees of mature New Zealand rabbits by fixing their left back knees straightenedly in plaster cast. The experimental animals received IFPUS therapy, of which the intensity was 2.0 W, and the frequency was 1 MHz. The IFPUS therapy was administered once a day and lasted for 15 minutes every time. For the control group, sham US therapy was administered. The immunohistochemistry was used to detect the expression of articular cartilage and synovial aquaporin 3 at 2, 4 and 8 weeks after therapy.RESULTS AND CONCLUSION: With the modeling time prolonged, the expression of articular cartilage and synovial aquaporin 3 in each group were increased. Compared with the control group, the expression of articular cartilage and synovial aquaporin 3 was lower in the experimental group (P ＜ 0.01). It is suggested that the low-frequency pulsed ultrasound might reduce the expression of aquaporin 3 in articular cartilage and synovial at protein level , relieve joint swelling, reduce the severity of articular cartilage and synovium degeneration, and have a protective effect on cartilage and synovial membrane.%背景:研究表明,低频脉冲超声能促进全层关节软骨缺损修复,延缓实验性早期骨关节炎的病变进展.水通道蛋白3存在于关节软骨及滑膜上,并在骨关节炎的发病机制中发挥重要作用.目的:探讨低频脉冲超声对实验性膝骨关节炎兔关节软骨及滑膜水通道蛋白3表达的影响.方法:成年新西兰兔左后膝关节用管型石膏固定于伸直位制动6周制备实验性兔膝关节骨关节炎模型,造模成功后随机分为两组:实验组予以低频脉冲超声治疗(频率为1 MHz,功率为2.0 W),1次/d,15 min/次;对照组不接受超声治疗.分别于治疗后2,4,8周,采用免疫组织化学方法观察兔关节软骨及滑膜水通道蛋白3的表达.结果与结论:随着造模后时间的延长,各组兔关节软骨及滑膜水通道蛋白3的表达均有所增加;与对照组比较,实验组兔关节软骨及滑膜水通道蛋白3表达明显降低(P < 0.01).说明低频脉冲超声能降低实验性兔膝骨关节炎关节软骨及滑膜水通道蛋白3的表达,从而缓解关节肿胀,延缓软骨及滑膜的退变,具有软骨及滑膜保护作用.