BACKGROUND: It is important to prepare a bone substitute material with good osteoacusis, osteoinductive activity and cell compatibility that is easily molded, which is of significance to repair irregular bone defects and refractory bone fractures.rnOBJECTIVE: To prepare demineralized bone matrix/sodium alginate (DBM/ALG) composite material and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility.rnMETHODS: DBM was prepared from the cortical bone of healthy donors by cleaning, grinding and demineralization. According to the ratio of 5/5, 6/4, 7/3, 8/2, the DBM was compounded with ALG, and the DBM/ALG composite material was prepared through screening the optimal ratio by disperse diameter method. L-929 fibroblasts of mice were cultured in the leaching liquor of DBM/ALG, and the cytotoxicity was tested by MTT method at days 2, 4 and 7.rnRESULTS AND CONCLUSION: With the increase of ALG content, the viscosity of DBM/ALG was strengthened and dispersed diameter was reduced gradually. When the ratio of DBM/ALG was 7/3, DBM/ALG was not discrete easily, and dispersed diameter was 3.7 cm in 37 癈 water. The results of MTT showed that cell proliferation rates were 91.56%, 95.43% and 97.47% at days 2, 4 and 7, respectively; while cytotoxicity was in grade 1.%背景:制备具有良好骨传导、骨诱导活性、细胞相容性,且可任意塑形的骨修复材料,对于不规则骨缺损以及难愈合性骨折的治疗具有重要意义.目的:构建可塑型脱矿骨基质/海藻酸钠复合组织工程骨材料,筛选出最佳复合比例并评价其细胞相容性.方法:选取健康供体皮质骨,经清洗、粉碎、脱钙处理制备脱矿骨基质,以5/5,6/4,7/3,8/2 比例与海藻酸钠复合,通过测定离散直径选取合适的比例构建脱矿骨基质/海藻酸钠复合材料.将所制备脱矿骨基质/海藻酸钠材料浸提液与L-929 小鼠成纤维细胞复合培养,2,4,7 d 后经MTT 法检测细胞毒性.结果与结论:随着海藻酸钠含量的增加,复合材料黏性变强,水中离散直径逐步减小,当脱矿骨基质/海藻酸钠质量比为7/3 时,复合材料可塑性强,不易离散,在37 ℃水浴中浸泡2 h 的离散直径为3.7 cm,为复合的最佳比例,经MTT 法检测,2,4,7 d 的细胞增殖率分别为91.56%,95.43%,97.47%,细胞毒性级别均为1 级.
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