仙灵骨葆对尾悬吊拟失重大鼠骨量丢失的抑制

摘要

背景:以淫羊藿苷为主要成分的中药制剂仙灵骨葆具有促进骨形成的作用.目的:观察仙灵骨葆对尾悬吊拟失重大鼠骨量丢失的抑制作用及机制.方法:将SD大鼠随机分为正常对照组、拟失重组、仙灵骨葆组,后两组大鼠采用尾悬吊法模拟失重,仙灵骨葆组悬吊同时灌胃给予仙灵骨葆250 mg/(kg?d),拟失重组悬吊同时灌胃给予双蒸水,4周后取右侧股骨行骨密度、三点弯曲生物力学及骨组织形态计量学检测.结果与结论:正常对照组骨密度、胫骨近端骨小梁相对体积、骨小梁厚度、骨小梁数量、生物力学最大载荷显著高于拟失重组、仙灵骨葆组(P < 0.05).仙灵骨葆组胫骨远端1/4骨密度、胫骨近端骨小梁相对体积显著高于拟失重组(P < 0.05),生物力学最大载荷也高于拟失重组,但差异无显著性意义.说明仙灵骨葆灌胃干预可促进松质骨的骨形成,部分阻止拟失重大鼠骨量的丢失.%BACKGROUND: Chinese herbal preparation Xianlinggubao whose main ingredient is icariin can promote bone formation. OBJECTIVE: To investigate the effect and related mechanism of Xianlinggubao on bone loss in tail-suspended rats. METHODS: Sprague-Dawley rats were divided into 3 groups: control group (A), simulated weightlessness group (B) and Xianlinggubao group (C). Rats in the group B and group C underwent tail-suspension, and in the group C was administered Xianlinggubao by gavage with dosage of 250 mg/(kg?d) at the same time while in the group B was administered double distilled water as control. Bone mineral density (BMD), biomechanical three-point bending and bone morphology of the right femurs were detected after 4 weeks removing the right femurs.RESULTS AND CONCLUSION: BMD, trabecular bone volume, bone trabecular thickness, trabecular number and biomechanics maximum load in the group A were markedly higher than those in groups B and C (P < 0.05), BMD of tibia distal 1/4, trabecular bone volume of proximal tibia in group C were higher as compared to group B (P < 0.05). Biomechanics maximum load in the group C was also higher than that of the group B, but there was no significant difference. Xianlinggubao inhibition by gavage can promote bone formation and partially prevent bone loss in tail-suspended rats.