腺病毒介导的骨形态发生蛋白基因增强在骨组织工程中的应用

摘要

BACKGROUND: The vector tool is needed when the target gene transduces into target cells, the vector can help the gene transduces into the cells and promote the protein expression. Vector selection is the key of the gene transduction. OBJECTIVE: To review research progress of the application of adenovirus medicated bone morphogenetic protein gene enhanced tissue engineering in osteogenic induction and repair of bone defects.METHODS: A computer-based search was performed on the PubMed database and CNKI database from January 1989 to January 2012 for the articles on the osteogenic induction of adenovirus medicated bone morphogenetic protein gene transfection and the articles on bone morphogenetic protein gene modified tissue engineered bone repair the bone defects. The key words were "adenovirus, bone morphogenetic protein, gene transfection, bone tissue engineering" in English and Chinese. The repetitive articles and the articles not in English or Chinese were eliminated, and finally, a total of 24 articles were included to review.RESULTS AND CONCLUSION: Adenovirus-mediated bone morphogenetic protein gene enhanced tissue engineering transfers the gene encoding with specific function factor to the seed cells using genetic engineering technology, which make the gene to produce the growth factor continuously. Transgenic cells stay on the surface of the stent and release the growth factor slowly, and can promote the proliferation and differentiation of osteoblast precursor cells and induce the directed differentiation of the osteoblast precursor cells in the bone defect area to osteoblasts, in order to repair the bone defects.%背景:将目的基因转导到靶细胞中需要载体工具,载体可协助基因进入细胞及表达蛋白,载体的选择是转基因成败的关键.目的:总结腺病毒介导的骨形态发生蛋白基因增强用于诱导成骨和修复骨缺损的研究现状.方法:应用计算机检索PubMed 数据库及中国期刊网全文数据库1989-01/2012-01 有关腺病毒介导骨形态发生蛋白基因转染的诱导成骨作用,骨形态发生蛋白基因修饰的组织工程化骨修复骨缺损的文章,英文检索词为"adenovirus,bone morphogenetic protein,gene transfection,bone tissue engineering",中文检索词为"腺病毒,骨形态发生蛋白,基因转染,骨组织工程".排除重复性及非中英文语种研究,共保留24篇文献进行综述.结果与结论:腺病毒介导的骨形态发生蛋白基因增强的组织工程利用基因工程技术将编码特定功能因子的基因转移到种子细胞上,使其持续产生生长因子.转基因后的细胞停留支架材料表面缓慢释放生长因子,促进成骨前体细胞增殖分化,也可在骨缺损处诱导成骨前体细胞向成骨细胞定向分化,从而修复骨缺损.