RECOMBINANT Adenovirus That Contains Ribozyme Mediating Trans-Splicing And The Anticancer Therapeutic Gene And Its Application

摘要

1. A recombinant adenovirus comprising: a polynucleotide encoding a trans-splicing mediated ribozyme complex and HSVtk (human herpes simplex virus thymidine kinase) that acts against TERT (telomerase reverse transcriptase) mRNA (SEQ ID NO: 1) representing cancer-specific gene; and anti-cancer therapeutic gene. 2. The recombinant adenovirus according to claim 1, where the polynucleotide encoding the complex contains the nucleotide sequence of SEQ ID NO: 2, which encodes the ribozyme Rib67, and the polynucleotide SEQ ID NO: 3, which encodes HSVtk. 3. The recombinant adenovirus according to claim 1, wherein the anticancer therapeutic gene is selected from the group consisting of a drug-activating gene, a proapoptotic gene, a cytostatic gene, a cytotoxic gene, a tumor suppressor gene, an antigenic gene, a cytokine gene, and an antiangiogenic gene. The recombinant adenovirus according to claim 3, wherein the anti-cancer therapeutic gene contains the nucleotide sequence of SEQ ID NO: 4, which encodes sPD-1 (soluble programmed death protein 1). A pharmaceutical composition for the prevention or treatment of cancer, comprising, as an active ingredient, the recombinant adenovirus according to any one of claims 1 to 4. The composition of claim 5, further comprising a pharmaceutically acceptable carrier, excipient or diluent. A method for treating cancer, comprising administering to a patient a pharmaceutically acceptable amount of a recombinant adenovirus according to any one of claims 1 to 4.