聚左旋乳酸/壳聚糖电纺丝纳米纤维支架与兔内皮祖细胞的生物相容性

摘要

背景 电纺丝技术能够使许多高分子材料制备出与细胞外基质相似的三维纳米纤维支架.聚乳酸/壳聚糖纳米纤维复合支架材料能够克服材料的不足,提高组织工程支架生物相容性.目的 评价聚左旋乳酸/壳聚糖电纺丝纳米纤维支架与兔内皮祖细胞的生物相容性.方法 电纺丝技术制备聚左旋乳酸,壳聚糖,聚左旋乳酸/壳聚糖的纳米纤维支架,扫描电镜观察其形貌结构.纳米纤维支架与内皮祖细胞进行复合培养后,观察细胞在不同材料上的黏附率、一氧化氮分泌,生长特征和在聚左旋乳酸/壳聚糖纳米纤维支架上的细胞表型特征.结果 与结论 聚左旋乳酸/壳聚糖纳米纤维支架比聚左旋乳酸、壳聚糖具有更合适的纤维直径,具有与细胞外基质相似的纳米纤维三维多孔结构.聚左旋乳酸/壳聚糖纳米纤维支架能够促进内皮祖细胞黏附率和细胞的一氧化氮分泌(P < 0.05,P <0.01).内皮祖细胞能够在聚左旋乳酸/壳聚糖复合材料膜上融合成片,保持了细胞的完整形态和分化功能,显示了内皮细胞特异性的vWF 表型.提示聚左旋乳酸/壳聚糖电纺丝纳米纤维支架与兔内皮祖细胞具有良好的生物相容性.%BACKGROUND: Three-dimensional nanofiber scaffold, similar to extracellular matrix, can be synthesized from many polymermaterials by electrospinning technique. Poly-L-lactic acid (PLLA)/chitosan (CS) nanofiber composite scaffold can overcome thedeficiency of each other to improve the biocompatibility of tissue engineering scaffold.OBJECTIVE: To evaluate biocompatibility of PLLA/CS electrospun nanofiber scaffold and rabbit endothelial progenitor cells(EPCs).METHODS: Nanofibrou scaffold of PLLA, CS and PLLA/CS were synthesized by electrospinning technology and morphologystructure was analysed by scanning electron microscope. After EPCs co-cultured with the nanofibers scaffold, adhere rates, NOproduction, morphological difference were investigated. Furthermore, phenotype of cell seeded on PLLA/CS was observed byimmunofluorescence.RESULTS AND CONCLUSION: PLLA/CS nanofibrous scaffolds have better diameter of nanometer fiber than the PLLA and CS.Three-dimensional porous nanofibrous structure similar to extracellular matrix was founded in PLLA/CS nanofibrous scaffolds.Adherence rate and NO production of EPCs were improved by PLLA/CS nanofibrous scaffolds (P < 0.05, P < 0.01 ). EPCs couldfuse with maintaining normal shapes and differentiation function in composite materials films of PLLA/CS. Cells in PLLA/CSexpressed vWF and then exhibited endothelial characteristic. An excellent biocompatibility in PLLA/CS electrospun nanofiberscaffold and rabbit endothelial progenitor cells.