大鼠RBL-2H3细胞瞬时感受器电位M7通道参与细胞存活及凋亡

摘要

BACKGROUND: Transient receptor potential melastatin 7 is an important calcium channel in mast cells. However, the role of transient receptor potential melastatin 7 in mast cell survival and apoptosis is unclear. OBJECTIVE: To investigate the role of transient receptor potential melastatin 7 in rat RBL-2H3 cells survival and apoptosis. METHODS: RBL-2H3 cells in logarithmic growth phase were intervened with 50, 100 and 200 μmol/L transient receptor potential channel blocker 2-APB. Cells intervened with 0.1% DMSO and normally cultured cells were used as control. RBL-2H3 cells were transfected with retrovirus vector of transient receptor potential melastatin 7-siRNA. Empty vector group and control group were constructed. RESULTS AND CONCLUSION: At 72 hour after treated with 100 and 200 μmol/L 2-APB, RBL-2H3 cell number was decreased. The optical density was decreased (P < 0.05). Cell apoptosis increased. Early stage apoptotic rate and total apoptotic rate increased (P < 0.05). At 72 hour after RBL-2H3 cells were transfected with transient receptor potential melastatin 7-siRNA, the optical density was decreased (P < 0.05). Cell apoptosis increased. Early stage apoptotic rate and total apoptotic rate increased (P < 0.05). These findings indicate that transient receptor potential melastatin 7 channel involve in the process of survival and apoptosis in RBL-2H3 cells.%背景:瞬时感受器电位M7是肥大细胞上重要的钙离子通道,但其在肥大细胞存活及凋亡过程中的作用仍未明确.目的:观察瞬时感受器电位M7通道对大鼠RBL-2H3细胞存活及凋亡的影响.方法:取对数生长期RBL-2H3细胞,①分别以50,100,200 μmol/L瞬时感受器电位通道阻断剂2-APB进行干预,并以0.1%DMSO干预的细胞及正常培养的细胞作对照.②以瞬时感受器电位M7-siRNA反转录病毒载体转染RBL-2H3细胞,并设立空载体组和正常对照组.结果与结论:经过100,200 μmol/L的2-APB作用72 h后,RBL-2H3细胞数减少,吸光度值降低(P < 0.05),细胞凋亡增多,早期凋亡率和总凋亡率增加(P < 0.05);RBL-2H3细胞转染si-瞬时感受器电位M7 72 h 后,吸光度值降低(P < 0.05),细胞凋亡增多,早期凋亡率和总凋亡率增加(P < 0.05).说明瞬时感受器电位M7通道参与了RBL-2H3细胞存活和凋亡过程.