肿瘤细胞三维培养支架、血管及细胞间的相互作用

摘要

背景：肿瘤组织工程通过构建综合的培养模型，充分模拟肿瘤在体内生长的微环境，可以较好地研究肿瘤发生发展的动力学及相关治疗策略。　　目的：综述肿瘤工程技术中的肿瘤细胞三维培养。　　方法：以“tumor engineering；3D culture；biological materials；dynamic”为关键词，检索PubMed数据库1992年1月至2013年3月相关文献，纳入有关肿瘤工程、肿瘤细胞三维培养、生物支架材料及肿瘤微环境的相关文章。　　结果与结论：三维培养因其可再现组织细胞的体内生长情况，已成为研究肿瘤耐药性、侵袭性和肿瘤微环境的重要平台，在许多领域表现出逐步取代平面培养技术的趋势，为肿瘤研究提供了一个非常接近于体内真实情况的研究平台。近年来，随着肿瘤工程学的发展，多种新型高分子聚合材料被应用于肿瘤细胞的三维立体培养，三维培养技术逐渐成为肿瘤生物学领域研究的热点，其利用各种方法及材料使细胞呈空间立体方式生长，形成类似体内生长环境的生物支撑或基质，建立细胞间及细胞与胞外基质间的相互联系，并形成特定的类似组织样的三维空间结构。生物材料就是种子细胞生长的土壤，在肿瘤工程中起着替代细胞外基质或组织、器官的基质的作用。而随着三维细胞培养技术在肿瘤研究中的广泛应用，其已成为肿瘤耐药、血管形成、细胞间相互作用、信号转导、干细胞等方面研究不可或缺的有力工具。%BACKGROUND:The tumor tissue engineering can build an integrated culture model to ful y simulate the in vivo microenvironment of tumor growth, which can be used to study tumor developmental dynamics and related treatment strategies. OBJECTIVE:To review the three-dimensional culture of tumor cells using tumor engineering technology. METHODS:PubMed database was retrieved for articles related to tumor engineering, three-dimensional culture of tumor cells, biological scaffold materials and tumor microenvironment published from January 1992 to March 2013. RESULTS AND CONCLUSION:Three-dimensional culture, because of its reproducible tissue and cellgrowth in vivo, has become an important platform for study of tumor resistance, invasiveness and tumor microenvironment. The three-dimensional culture has showed a trend to gradual y replace the flat culture technique in many fields, and provides a research platform which is very close to in vivo environment. In recent years, with the development of tumor engineering, a variety of new polymer materials have been used in the three-dimensional culture of tumor cells. Three-dimensional culture technology is becoming a hotspot in the field of tumor biology, in which, using a variety of methods and materials, the cells show a growth in the spatial manner to form a biological support or matrix similar to in vivo growth environment. Biomaterials have become the soil on which seed cells can grow wel , and plays an alternative to the extracellular matrix or the matrix of tissues and organs in the tumor engineering. Therefore, the three-dimensional cellculture has been widely used in cancer research, which has become a powerful tool to tumor drug resistance, angiogenesis, cel-cellinteraction, signal transduction, stem cells and other research.