附子理中汤及拆方干预脾阳虚模型大鼠**☆

摘要

背景：中药复方多具有“双向调节”、“纠正偏性”从而恢复机体平衡的药效特点，实验拟采用附子理中汤及其拆方为干预药物，验证附子理中汤对脾阳虚证具有最佳的“温阳健脾”效果，为进一步了解附子理中汤多节点起效的机制提供基础资料。　　目的：以转化医学思维为指导观察附子理中汤及拆方对脾阳虚模型大鼠的药效。　　方法：采用肩胛间棕色脂肪切除术+高脂饲料喂养+隔日寒冷环境刺激的复合因素造模法制备脾阳虚Wistar大鼠模型，造模后第70天分别灌胃附子理中汤、理中汤和附子干姜汤；对照组不造模，喂饲普通饲料，测量各组大鼠体质量、肛温和肛温温度曲线下面积。　　结果与结论：模型组大鼠体质量增长最慢，其余给药3组体质量增长较快，又以附子组增长最明显，与模型组比较，差异有显著性意义(P <0.05)。模型组大鼠肛温均低于其余4组，与对照组比较，差异有显著性意义(P<0.05)。附子组大鼠肛温升高明显，与模型组比较差异有显著性意义(P<0.05)。模型组大鼠的肛温温度曲线下面积低于对照组，差异有显著性意义(P <0.05)。附子组、理中组、附姜组大鼠的肛温温度曲线下面积高于模型组，差异有显著性意义(P<0.05或P<0.01)。结果可见附子理中汤以及拆方理中汤和附子干姜汤均能纠正脾阳虚大鼠脾失健运导致体质量减少或增长缓慢和脾失温煦导致体温低的病理状态，以附子理中汤的效果较好。%BACKGROUND: Chinese herbal compounds mostly pose pharmacodynamic characteristics of “two-way adjustment”and“corrective bias”to restore the body’s balance. This experiment is proposed to verify the effect of Fuzi Lizhong Decoction and its disassembled prescription on deficiency of spleen-yang syndrome via warming yang and invigorating the spleen, further providing basic data for understanding the multi-node mechanism. OBJECTIVE:To observe and evaluate the curative effects of Fuzi Lizhong Decoction and its disassembled prescription in the treatment of rats with spleen-yang deficiency syndrome under the conception of translational medicine. METHODS:A model of deficiency of spleen-yang syndrome was made in Wistar rats through interscapular removal of brown fat and high fat diet under cold environment that changed every other day. After 70 days, the rat models were administered with Fuzi Lizhong Decoction, Lizhong Decoction and Fuzi Ganjiang Decoction, respectively. Another Wistar rats without modeling were used as controls that were fed with normal diet. Body mass, rectal temperature and area under rectal temperature curve were measured in al the groups. RESULTS AND CONCLUSION:Body mass was increased slowest in the model group and fastest in the Fuzi Lizhong Decoction group (P<0.05). The model group had lower rectal temperature than the other four groups and significantly differed from the control group in the rectal temperature (P<0.05). There was a significant difference in the rectal temperature between the Fuzi Lizhong Decoction and model groups (P<0.05). Area under rectal temperature curve in the model group was lower than that in the control group (P<0.05). As compared with the models group, Fuzi Lizhong Decoction, Lizhong Decoction and Fuzi Ganjiang Decoction groups had the larger area under the rectal temperature curve (P<0.05 or P<0.01). Fuzi Lizhong Decoction, Lizhong Decoction and Fuzi Ganjiang Decoction can al improve the pathological conditions of rats with spleen-yang deficiency syndrome presenting with body mass reduction or slow growth and low temperature. Fuzi Lizhong Decoction has the best biologic effects among these three decoctions.