钛基表面纳米银复合涂层体外抗菌活性及生物安全性评价

摘要

BACKGROUND:Hydroxyapatite is the main component of bone with the capacity of osteogenic induction. Nano-silver has broad-spectrum and efficient antibacterial activity, while hydroxyapatite/nano-silver composite coating on the surface of titanium base has either biological activity or antimicrobial properties. OBJECTIVE:To study the antimicrobial properties of hydroxyapatite/nano-silver composite coating on the surface of titanium base and to evaluate the biocompatibility and security of hydroxyapatite/nano-silver composite coating on the surface of titanium base. METHODS: Silver solution of 0, 0.5, 1.0 mmol/L was selected to prepare hydroxyapatite/nano-silver composite coating samples deposited on the surface of titanium base. Then,Staphylococcus aureus was cultured in the sample leaching solution for thein vitro antibacterial qualitative analysis. In accordance with national and international standardization for biocompatibility testing, healthy adult Kunming mice and New Zealand rabbits were subjected to pyrogen test, hemolysis test, acute toxicity test, skin irritation test for comprehensive evaluation of the material biocompatibility and safety. RESULTS AND CONCLUSION:The hydroxyapatite/nano-silver composite coating on the surface of titanium base exhibited obvious antibacterial activityin vitro againstStaphylococcus aureus, in a silver dose-dependent manner. There was no pyrogen response, hemolytic reaction, acute toxic reaction and skin stimulus response in the animal experiments. There was also no difference in the biocompatibility of hydroxyapatite materials containing silver or not. These findings indicate that the hydroxyapatite/nano-silver composite coating on the surface of titanium base has good antibacterial activity for Staphylococcus aureusin vitro as wel as excelent biocompatibility.%背景：羟基磷灰石是骨的主要成分，具有诱导成骨细胞的功能。纳米银具有广谱和高效的抗菌效果，钛基表面羟基磷灰石/纳米银复合涂层材料既有生物活性又具有抗菌特性。　　目的：研究钛基表面羟基磷灰石/纳米银复合涂层体外抗菌作用及影响因素，评价钛基表面羟基磷灰石/纳米银复合涂层的生物相容性和安全性。　　方法：选取浓度0，0.5，1.0 mmol/L银溶液制备钛基表面沉积的羟基磷灰石/纳米银复合涂层样品，将其浸提液与金黄色葡萄球菌与材料共培养，进行体外抗菌定性分析。按照国家和国际标准化组织对生物材料相容性检测标准，对健康成年昆明小鼠、新西兰兔进行热原试验、溶血试验、急性毒性试验、皮肤刺激试验，综合评价材料的生物相容性和安全性。　　结果与结论：钛基表面沉积制备的羟基磷灰石/纳米银复合涂层材料体外对金黄色葡萄球菌有明显抗菌作用，复合材料中银含量越高抗菌作用越明显，且动物实验中未出现明显的热原反应、溶血反应、急性毒性反应、皮肤刺激反应，与不含银的纯羟基磷灰石材料相比，生物相容性差异无显著性意义。说明钛基表面沉积制备的羟基磷灰石/纳米银复合材料，体外对金黄色葡萄球菌具有良好的抗菌作用，且具有良好的生物相容性。