背景：脱细胞纤维环基质和纤维环干细胞均来源于纤维环组织，二者复合构建的组织工程复合物可能具有更好的生物相容性。 目的：将兔的纤维环干细胞和猪的脱细胞纤维环基质进行体外共培养，观察细胞在脱细胞基质支架上的生长状态。 方法：制备猪脱细胞纤维环基质，通过扫描电镜和 DAPI 染色观察材料制备效果，傅里叶红外光谱仪鉴定基质的成分；分离和培养兔纤维环干细胞，将第1代纤维环干细胞种植于脱细胞纤维环基质表面，行细胞骨架染色，倒置免疫荧光显微镜和扫描电镜下观察细胞形态，并绘制细胞生长曲线。 结果与结论：制备成的猪脱细胞纤维环基质呈白色半透明状，扫描电镜下为纤维网状结构，DAPI染色显示无细胞残留，红外光谱分析显示脱细胞纤维环基质主要成分是胶原蛋白。细胞在支架材料上生长第1，3，7天的细胞骨架染色显示细胞很好的黏附于支架表面，生长状态良好，表明脱细胞纤维环基质有着良好的生物相容性，纤维环干细胞在其表面生长良好。%BACKGROUND:Acellular matrix and annulus fibrosus-derived stem cells are both derived from the annulus tissue, and their tissue engineering complexes may have better biocompatibility. OBJECTIVE:To culture rabbit annulus fibrosus-derived stem cells on the porcine decellularized annulus fibrosus matrix, and to observe the growth of annulus fibrosus-derived stem cells on the decellularized matrix scaffold. METHODS:Decellularized annulus fibrosus matrix from porcine was prepared and detected by scanning electron microscopy, DAPI staining and Fourier transform infrared spectroscopy analysis. After isolation and culture, annulus fibrosus-derived stem cells were seeded onto the decellularized annulus fibrosus matrix. cellgrowth on the scaffolds was observed by cytoskeleton staining, inverted immunofluorescence microscopy and scanning electron microscopy to draw cellgrowth curve. RESULTS AND CONCLUSION:The prepared decellularized annulus fibrosus matrix was white and translucence liquid. The result of Fourier transform infrared spectroscopy indicated that the main component of the decellularized annulus fibrosus matrix was col agen. DAPI staining and scanning electron microscopy results showed no cells resident. The cytoskeleton staining displayed that annulus fibrosus-derived stem cells grew wel on the scaffolds at 1, 3, 7 days. These findings indicated that annulus fibrosus-derived stem cells have a good biocompatibility with the decellularized annulus fibrosus matrix in vitro.
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