白细胞介素10与沙门菌接合性质粒介导细菌生物膜的形成

摘要

背景：既往研究发现接合性质粒pRST98可促进其宿主菌生物膜的形成，携带pRST98的菌株感染细胞和实验动物后，可促进白细胞介素10的分泌和表达。　　目的：体外研究不同质量浓度白细胞介素10与接合性质粒pRST98对鼠伤寒沙门菌生物膜形成的相互影响。　　方法：体外建立鼠伤寒沙门菌标准株χ3306、突变株χ3337及接合株χ3337/pRST983组生物膜模型，3组分别加入0(空白对照)，1，10，100µg/L白细胞介素10，通过结晶紫染色半定量法、共聚焦激光扫描显微镜分析和扫描电镜观察，比较不同质量浓度白细胞介素10对携带和不携带接合性质粒 pRST98的鼠伤寒沙门菌生物膜形成的影响。　　结果与结论：①3组组内比较：与空白对照组比较，1，10µg/L白细胞介素10均能促进鼠伤寒沙门菌的聚集，提高生物膜形成能力，且10µg/L质量浓度效果更明显；100µg/L白细胞介素10抑制鼠伤寒沙门菌生物膜的形成。②3组组间比较：在同一白细胞介素10质量浓度下，接合株χ3337/pRST98组 A570高于标准株χ3306组、突变株χ3337组。结果说明1，10µg/L白细胞介素10可促进生物膜的形成，且在携带接合性质粒pRST98的情况下促进作用更明显。%BACKGROUND:Previous studies discovered that pRST98, original y isolated from Salmonel a enterica serovar typhimurium (S.typhimurium) could promote bacterial biofilm formation. In addition, bacterial harboring pRST98 can promote the secretion and expression of interleukin-10 after infection in cells and animals. OBJECTIVE:In vitro studies have discovered the effects of interleukin-10 at varying concentrations and conjugative plasmid pRST98 on the biofilm formation of S.typhimurium. METHODS:S.typhimurium wild-type strainχ3306, virulence plasmid-deletion S.typhimurium strainχ3337 and pRST98-transconjugant S.typhimuriumχ3337/pRST98 were established in vitro and cultured for biofilm formation. 1, 10, 100 µg/L interleukin-10 were added during the biofilm formation. 0 µg/L interleukin-10 was set as a control. Crystal violet staining method, semi-quantitative method, confocal laser scanning microscopy and scanning electron microscopy were used to determine the effects of interleukin-10 on the biofilm formation and compare the effects of S.typhimurium with or without pRST98. RESULTS AND CONCLUSION:Intra-group comparison showed that, compared with the control group, S.typhimurium gathered together and formed thicker biofilm in concentration of 1 and 10 µg/L of interleukin-10. The promotion effects of S.typhimurium on biofilm formation were greatly improved in 10 µg/L. Interleukin-10 in 100 µg/L inhibited S.typhimurium biofilm formation. Inter-group comparison showed that, A570 inχ3337/pRST98 was greatly higher than that inχ3306 andχ3337 under the same concentration of interleukin-10. The results indicate that both 1 and 10 µg/L of interleukin-10 promote biofilm formation, especial y bacteria harboring pRST98.