人脐带间充质干细胞的分离鉴定及多向诱导分化

摘要

背景：脐带间充质干细胞取材方便、无创，不受伦理学限制，比一般干细胞原始，免疫原性小，其应用前景广阔，是一种理想的种子细胞。　　目的：分离鉴定脐带间充质干细胞，并诱导其向成骨细胞和成脂细胞分化。　　方法：组织块贴壁法分离纯化脐带间充质干细胞，取对数生长期的第3代细胞，观察细胞形态、生长方式；流式细胞仪检测干细胞表型CD90、CD105、CD34和CD45的表达情况，并在体外检测能否将其诱导分化为成脂细胞及成骨细胞。　　结果与结论：用组织块法成功分离培养出脐带间充质干细胞，流式细胞学鉴定显示细胞强表达 CD90和CD105，不表达 CD34和 CD45；能在体外将其成功诱导为脂肪细胞和成骨细胞。结果显示组织块贴壁法能够从人脐带中分离出间充质干细胞，该细胞可向成脂细胞及成骨细胞分化。%BACKGROUND:The umbilical cord mesenchymal stem cels can be obtained conveniently, noninvasively, without ethical restrictions, which are more original that stem cels from other sources and have little immunogenicity. Therefore, umbilical cord mesenchymal stem cels are a kind of ideal seed cels with promising application prospects. OBJECTIVE: To induce the differentiation of mesenchymal stem cels derived from human umbilical cord into adipocytes and osteoblasts. METHODS: The umbilical cord mesenchymal stem cels were isolated by tissue explants method. The morphology, proliferation and immunophenotype of the 3rd passage cels were analyzed, and then cels were induced to adipocytes and osteoblastsin vitro. The expressions of CD90, CD105, CD34 and CD15 were detected by flow cytometry. RESULTS AND CONCLUSION:The umbilical cord mesenchymal stem cels isolated by tissue explants method could be cultured and proliferatedin vitro. Flow cytometry analysis revealed that the cels were strongly positive for CD90 and CD105, but negative for CD34 and CD45. The umbilical cord mesenchymal stem cels could be induced to adipocytes and osteoblastsin vitro. These findings indicate that mesenchymal stem cels can be successfuly obtained from human umbilical cord by tissue explants method and have the potential of multi-directional differentiation.