背景：胰岛素样生长因子1具有促进成纤维细胞有丝分裂的作用，同时具有促进牙周细胞生长、分化及合成细胞外基质的作用。 目的：观察负载胰岛素样生长因子1的壳聚糖胶原支架对于人牙周膜细胞增殖的作用。 方法：将人牙周膜细胞分别接种于负载胰岛素样生长因子1的壳聚糖胶原支架与普通胶原支架上，于接种的1 h、24 h及1周检测重组人转化生长因子β1的释放，于第1，7，28天检测两组细胞的黏附和增殖情况。结果与结论：负载胰岛素样生长因子1的壳聚糖胶原支架组第1，24小时和第1周的重组人转化生长因子β1释放率明显低于普通胶原支架组(P <0.01)。两组接种第1天的细胞黏附和增殖检测比较差异无显著性意义(P >0.05)，负载胰岛素样生长因子1的壳聚糖胶原支架组接种第7，28天的细胞黏附和增殖情况优于普通胶原支架组(P <0.01)。表明负载胰岛素样生长因子1的壳聚糖胶原支架可显著促进人牙周膜细胞的增殖。%BACKGROUND:Insulin-like growth factor-1 can promote mitosis of fibroblasts as wel as periodontal cellgrowth, differentiation and synthesis of extracelular matrix. OBJECTIVE: To observe the effects of chitosan/colagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cels. METHODS: The human periodontal ligament cels were seeded on chitosan/colagen composite scaffold carrying insulin-like growth factor-1 and ordinary colagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; celladhesion and proliferation were detected at days 1, 7 and 28. RESULTS AND CONCLUSION:The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary colagen scaffold at 1, 24 hours and 1 week after cellseeding (P < 0.01). The celladhesion and proliferation showed no difference between two groups at day 1 after cellseeding, but became significantly higher in the composite scaffold than that in the ordinary colagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/colagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cels.
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