BACKGROUND:Laminectomy for decompression can effectively play an effect on spinal nerve decompression. However, this operation can induce epidural fibrosis and fibers broke into the spinal canal, result in iatrogenic spinal stenosis, persistent or recurrent leg pain. Chlorogenic acid is one of the effective pharmacological ingredient of honeysuckle, and has anti-inflammatory effects. n OBJECTIVE:To observe the effects of topical application of chlorogenic acid on epidural fibrosis and dural adhesion in rat models of laminectomy. METHODS:A total of 72 healthy adult Wistar rats were selected to prepare models of laminectomy, and randomly assigned to three groups (n=24). In the chlorogenic acid group, rats were administered chlorogenic acid 2 mL/rat before skin suture in the laminectomy area. Rats in the physiological saline group were given an equal volume of physiological saline. Rats in the blank control group were not given any treatment. Rats in each group were sacrificed at 4 weeks after model establishment. The general assessment and histological analysis were conducted. Hydroxyproline contents, interleukin-6 levels, transforming growth factor-β1 expression were measured. RESULTS AND CONCLUSION:Rats in each group were involved in the final analysis, dissected and sampled. In the chlorogenic acid group, less epidural col agen fibers proliferated and the appearance of fibers was normal. In the physiological saline and blank control groups, many epidural col agen fibers proliferated, and their appearance showed obvious scar tissue. Histological evaluation exhibited that the density of fibroblasts was lower in the chlorogenic acid group than in other two groups. Hydroxyproline contents of epidural fibrous scar were significantly lower in the chlorogenic acid group than in the physiological saline and blank control group (P<0.01). RT-PCR results demonstrated that interleukin-6 levels and transforming growth factor-β1 expression were lower in the chlorogenic acid group than in the other two groups. These data indicated that the topical application of chlorogenic acid in rat models of laminectomy could inhibit fibroblast proliferation, reduce interleukin 6 and transforming growth factor-β1 expression, and prevent epidural scar adhesion.%背景：椎板切除减压能起到有效的脊髓神经减压作用，但该操作也会发生硬膜外纤维增生并突入椎管，致医源性椎管狭窄，发生持续或复发的腰腿疼痛。绿原酸是金银花的有效药理成分之一，主要有抗炎等作用。n 目的：观察局部应用绿原酸对椎板切除模型大鼠硬膜外纤维化及硬脑膜粘连的影响。n 方法：选用72只健康成年Wistar大白鼠，制备椎板切除模型，随机分成3组(n=24)。绿原酸组皮肤缝合前椎板切除区域给绿原酸溶液2 mL/只；生理盐水组给予等量的生理盐水；空白对照组术后不做任何处理。各组大鼠均于造模后4周被处死，进行实验评估，包括大体评价、组织学分析、羟脯氨酸含量测定及白细胞介素6、转化生长因子β1的表达情况。n 结果与结论：3组大白鼠均进入结果分析，解剖并取样。绿原酸组硬膜外胶原纤维增生较少，外观较为正常；生理盐水组和空白对照组硬膜外胶原纤维明显增多，外观可见明显纤维瘢痕组织。组织学评价显示，绿原酸组的组织染色切片中成纤维细胞的密度明显比其他两组的成纤维细胞密度低。绿原酸组硬膜外纤维瘢痕的羟脯氨酸含量显著低于生理盐水组和空白对照组(P <0.01)。通过RT-PCR方法测得绿原酸组白细胞介素6和转化生长因子β1的表达低于另外两组。提示椎板切除模型大鼠局部应用绿原酸可以抑制成纤维细胞增殖，同时可以降低白细胞介素6和转化生长因子β1的表达，防止硬膜外瘢痕粘连。
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