背景：在临床工作中，制取口腔印模时必然与患者的唾液、黏膜甚至血液直接接触，印模表面经常附有多种病原微生物，因此，对印模进行消毒灭菌处理不容忽视。目的：检测戊二醛、二氧化氯、氧化电位水对印模的消毒效果，研究消毒对印模材尺寸稳定性的影响，为建立标准、合理的消毒方法提供依据。方法：应用戊二醛、二氧化氯、氧化电位水分别对体外人工染菌的藻酸盐印模进行不同时间(5，10，15 min)的浸泡消毒，通过细菌培养、菌落计数检测消毒效果，比较各消毒剂浸泡不同时间的消毒效果，消毒液浸泡后翻制超硬石膏模型，通过高精度结构光扫描，重建三维数字化模型，进行全局偏差测量分析和指标测量，比较不同消毒方法对模型表面精度及尺寸稳定性的影响。结果与结论：2%碱性戊二醛浸泡5 min对实验菌达到100%杀菌率，浸泡10 min对乙肝病毒有效杀灭；600 ppm二氧化氯浸泡15 min对实验菌达到99%以上杀灭，同时对乙肝病毒有效杀灭；氧化电位水浸泡15 min杀菌率不足99%，对乙肝病毒不能有效杀灭。3种消毒剂浸泡印模不同时间后灌注石膏模型，重建后的三维数字化模型，各组间尺寸变化无统计学差异。提示2%碱性戊二醛浸泡10 min和600 ppm二氧化氯浸泡15 min可以对藻酸盐印模特定染菌达到有效消毒，且消毒对印模尺寸稳定性没有明显影响。%BACKGROUND:The surface of dental impression is inevitable to carry various bacteria caused by direct contact with the patient saliva, mucosa and blood during preparation;therefore, disinfection of the dental impression is necessary. OBJECTIVE:To evaluate the disinfection efficiency of glutaraldehyde, chlorine dioxide and electrolyzed oxiding water on dental impressions and to investigate the dimensional stability of impressions after disinfection, thus providing basis for establishing a standard and reasonable disinfection method. METHODS:Alginate impression materials were contaminated in vitro, and then immersed in glutaraldehyde, chlorine dioxide and electrolyzed-oxiding water for 5, 10 and 15 minutes, respectively. The colonies were counted after germiculture to compare the disinfection efficiency of three disinfectants. The impressions were poured in die stone after immersion, the dental models were structured-light scanned and three-dimensional digital dental models were reconstructed. Al the data were global y registered, and linear dimensions were measured on the digital models to deduce the influence of disinfection on surface accuracy and dimensional stability of the impressions. RESULTS AND CONCLUSION:The disinfecting rate reached 100%through immersion in 2%alkaline glutaraldehyde for 5 minutes, and the disinfection of kil ing the hepatitis B virus was effective after 10-minute immersion. The disinfecting rate of 600 ppm chlorine dioxide for 15 minutes kil ing experimental bacteria reached 99%and effective for kil ing the hepatitis B virus. While the disinfecting rate of kil ing experimental bacteria was less than 99%through immersion in electrolyzed oxiding water for 15 minutes, and was not effective for hepatitis B virus. The three-dimensional digital dental models did not differ significantly. These results suggest that immersion in 2%alkaline glutaraldehyde for 10 minutes or 600 ppm chlorine dioxide for 15 minutes can effectively disinfect alginate impressions and make no significant effect on the dimensional stability.
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