背景:人尿源性干细胞研究较少,目前尚无稳定高效的培养方法.目的:比较3种培养基对人尿源性干细胞生长和增殖的影响,优化其培养方法.方法:离心、提纯尿液获取人尿源性干细胞,利用贴壁法分别在角质细胞无血清培养基、祖细胞培养基以及尿源性细胞培养基(角质细胞无血清培养基和祖细胞培养基以等比例混合而成)进行培养,观察细胞生长状态, MTT法检测细胞增殖情况,绘制生长曲线.结果与结论:3种培养基均能够培养出尿源性干细胞,尿源性干细胞在角质细胞无血清培养基中缓慢生长,在祖细胞培养基中生长较快,在尿源性细胞培养基中生长最好.结果表明,尿源性细胞培养基为人尿源性干细胞最佳生长培养基,能够快速、高效的培养出人尿源性干细胞.%BACKGROUND: Little is reported on human urine derived stem cells, and there is still no stable and efficient culture method until now. OBJECTIVE: To compare the effects of three kinds of culture media on the growth and proliferation of human urine derived stem cells, and to optimize the culture methods. METHODS: Human urine derived stem cells were isolated and purified using centrifugation method, and then cultured using adherent method in keratinocyte serum-free medium, progenitor cell culture medium, and urinary cell culture medium (equal-proportion mixture of keratinocyte serum-free medium and progenitor cell culture medium). Cell morphology was observed, and cell proliferation was detected by MTT method to draw a cell growth curve.RESULTS AND CONCLUSION: Human urine derived stem cells could be successfully cultured in these three kinds of culture media, which grew slowly in the keratinocyte serum-free medium, grew rapidly in the progenitor cell culture medium, and grew best in the urinary cell culture medium. To conclude, the urinary cell culture medium which can rapidly and efficiently culture target cells is the best choice for the growth of human urine derived stem cells.
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