核因子κB经典信号转导通路在糖尿病患者桡动脉中的表达及定位

摘要

Objective To observe the expression and localization of the nuclear factor-κB (NF-κB) classic signaling pathway in the radial artery of the coronary artery bypass grafting (CABG) patients with diabetes and non-diabetes.Methods Samples of radial artery from 36 cases of diabetic and non-diabetic patients were randomly collected from January 2012 to December 2012.In the diabetic group there were 13 male cases,5 female cases,with an average age of (69 ± 6) years.In non-diabetes group there were 13 male cases,5 female cases,with an average age of (70 ± 6) years.HE staining techniques was used to test the morphology of radial artery.Immunohistochemical techniques was then used to test the expression and localization of key factors (inhibitor of kappa B kinase β (IKKβ),P50,P65,tumor necrosis factor-α (TNF-α) and endothelial nitric oxide synthase (eNOS)) in the NF-κB classical signaling pathway in the two groups of patients.Results The radial artery intima was signifiantly thickened in the diabetic patients when compared with non-diabetic patients by HE staining.And there wss a lot of inflammation and foam cell infiltration in the radial artery intima of the diabetic patients.Using immunohistochemical techniques the expression of IKKβ,P50,P65 in diabetic group were obviously higher than non-diabetic group (4.4 ± 0.5vs.1.1±0.6,4.8±0.7vs.1.2±0.7,4.2±0.4vs.1.6±0.7,t=18.238,15.052,13.535,all P ＜ 0.01).In the aspect of inflammatory cytokines,the expression of TNF-α and eNOS factors in diabeics group were significantly higher than non-diabetic group (5.5 ± 0.5 vs.1.4 ± 0.7,3.3 ± 0.5 vs.0.8 ± 0.4,t =20.118,16.764,all P ＜0.01)also.And the five kinds of protein were mainly located in radial artery intima layer cells in diabetic patients.Conclusions Expression levels of 5 key factors in NF-κB classic signaling pathway are significantly higher in radial artery intimal layer of diabetic patients than non-diabetic patient's.And Positive signals are more concentrated in the radial artery intima layer of cells.%目的 观察核因子κB经典信号转导通路在糖尿病及非糖尿病冠状动脉旁路移植术(CABG)患者桡动脉中的表达及定位情况.方法 收集2012年1月至12月接受CABG治疗的36例患者的桡动脉标本,按照是否患有糖尿病分为两组.糖尿病组男性13例,女性5例,平均年龄(69±6)岁;非糖尿病组男性13例,女性5例,平均年龄(70±6)岁.利用HE染色比较两组桡动脉管壁的形态,并通过免疫组化染色比较核因子κB经典信号转导通路关键因子[核因子κB抑制蛋白激酶(IKKβ)、P50、P65、肿瘤坏死因子α(TNF-α)、内皮型一氧化氮合酶(eNOS)]在两组患者桡动脉中的表达量及定位情况.结果 HE染色结果显示糖尿病组桡动脉内膜较非糖尿病组明显增厚,且有大量炎症和泡沫细胞浸润.免疫组化染色结果显示糖尿病组IKKβ、P50及P65表达量高于非糖尿病组(4.4±0.5比1.1±0.6,4.8±0.7比1.2±0.7,4.2±0.4比1.6±0.7,t=18.238、15.052、13.535,均P＜0.01);在炎症因子方面,糖尿病组TNF-α及eNOS的表达量也高于非糖尿病组(5.5±0.5比1.4±0.7,3.3±0.5比0.8±0.4,=20.118、16.764,均P＜0.01).5种蛋白均主要定位于糖尿病组患者桡动脉内膜层的细胞上.结论 糖尿病患者桡动脉内膜层中核因子κB经典信号转导通路中的5个关键因子的表达水平明显高于非糖尿病组患者,并且多定位于桡动脉内膜层的细胞.