Objective To observe the expression changes of signal transduction pathway of enteric nerne system-interstitial cell (ENS-ICC) and the therapeutic effects of Xiang Sha Liu Jun Zi decoction(XSLJZD) in rats with deficiency of Spleen-Qi syndreme (DSQS). Methods One hundred Wistar rats were randomly divid-ed into control group, DSQS group, recovery group and XSLJZD group. The signal transduction pathway of ENS-ICC of the gastrointestinal tract was observed by using immunohistochemical double-staining with whole-amount preparation technique and confocal laser scanning microcopy. Results The IOD of ICC of intes-tine in DSQS group was significantly lower than that in control group(P<0.01), the ICC network was significant-ly disrupted. The IOD of ICC of intestine in the recovery group was slightly better than that in DSQS group(P<0.01), the ICC network was slightly recovered. The IOD of ICC of intestine in XSLJZD treatment group was sig-nificantly increased(P<0.01). After treatment with XSLJZD, the ICC network was significantly recovered. The IOD of protein kinase A(PKA)/protein kinase C(PKC) in DSQS group was significantly decreased(P<0.01). The signal transduction pathway was destroyed. The IOD of protein kinase A(PKA)/protein kinase C(PKC) in the re-covery group was slightly recovered(P<0.01). While the IOD of protein kinase A(PKA)/protein kinase C(PKC) in XSLJZD treatment group was significantly increased. The signal transduction pathway was significantly recov-ered. Conclusion XSLJZD can improve the gastrointestinal motility in DSQS and the mechanism may be re-lated to the repairing effect on the distribution and the connection of Signal Transduction Pathway of ENS-ICC in rat with DSQS.% 目的：观察脾气虚证大鼠肠神经Cajal间质细胞(ICC)间信号转导通路的表达改变和香砂六君子汤的治疗作用。方法：将100只Wistar大鼠随机分为对照组、模型组、自然恢复组和香砂六君子汤治疗组，采用免疫荧光双标记染色、激光扫描共聚焦显微镜观察模型组大鼠小肠肠神经-ICC信号转导通路损伤和香砂六君子汤的修复作用。结果：脾气虚证大鼠小肠ICC计数和荧光强度（IOD）值明显降低，网络样结构遭到破坏，PKA/PKC蛋白荧光强度IOD值明显降低。结论：香砂六君子汤能够修复脾气虚证大鼠小肠Cajal间质细胞和信号转导通路的损伤，改善胃肠运动障碍，治疗脾气虚证。
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