复方莪术散对大鼠子宫内膜异位症基质细胞衍生因子-1及其受体表达的影响

摘要

Objective To investigate the influence of compound Ezhu powder on stromal cell derived fac⁃tor-1 (SDF-1) and its receptor CXC chemokine receptor (CXCR4) in endometriosis (EM) rats model. Methods The EM model were established by self-endometrium transplantation during non-estrus SD rats. Af⁃ter 21 days of establishing EM model successfully, the EM rats were divided into the compound Ezhu powder high-dose group, middle-dose group, low-dose group and model group (each group of 10 rats) and received cor⁃responding traetment.. After 28 days, the mRNA expression of SDF-1 and CXCR in ectopic endometriotic tissue were observed by PCR. Results The mRNA expression of SDF-1 and CXCR4 in ectopic endometrial tissue was significantly increased compared with eutopic endometrium and normal endometrium(P<0.05). In each treatment group, The SDF-1mRNA expression of ectopic endometrial tissue was decreased, compound Ezhu pow⁃der high-dose group were significantly lower in comparison wtith the middle-dosegroup and the small-dose group (P<0.05). In each treatment group, CXCR4mRNA expression was decreased, compound Ezhu powder high-dose group and middle-dose group have low expression of CXCR4 mRNA , there exist significant differ⁃ence in comparison with the model group. Conclusion SDF-1 and CXCR4 expression were significantly stronger in the ectopic endometrium group than that in the eutopic endometrium group. The increased expression of SDF-1 and CXCR4 in the ectopic endometriosis tissue of EM rats might be had relationship with the patho⁃genesis of endometriosis. Compound Ezhu powder could inhabit the growth of ectopic endometrium in EM rats. Its mechanism might be associated with re⁃ducing the expression of SDF-1and CXCR4 of ecto⁃pic endometriotic tissue.%目的：观察子宫内膜异位症大鼠异位内膜组织基质细胞衍生因子-1（SDF-1)及其趋化因子受体（CXCR4）的表达情况，并探讨复方莪术散对其表达的影响。方法：自体移植法建立非动情期SD大鼠子宫内膜异位症模型，PCR法检测大鼠在位、异位内膜组织及正常在位子宫内膜组织SDF-1和CXCR4的mRNA表达，分析各组统计学差异；造模21 d后，分组连续给药28 d，对比观察中药复方莪术散（高、中、低剂量组各10只）与模型组（10只）异位症内膜组织中SDF-1 mRNA，CXCR4 mRNA的表达差异。结果：SDF-1 mRNA和CXCR4 mRNA在大鼠异位内膜组织高表达，与在位内膜组织及正常子宫内膜组织比较有统计学差异（P＜0.05）；各治疗组异位内膜组织SDF-1mRNA表达均下降，高剂量组(0.646±0.264）与中、低剂量组（1.001±0.160、1.714±0.343）比较降低明显，且各组间比较有统计学意义（P＜0.05）；各治疗组CXCR4 mRNA表达有下降趋势，复方莪术散高剂量(0.587±0.476)和复方莪术散中剂量(1.408±0.252)组CXCR4 mRNA表达分别与模型组(3.004±0.264)比较有统计学差异（P＜0.05）。结论：SDF-1、CXCR4在大鼠在位内膜组织中的表达高于正常子宫内膜组织，其表达可能与大鼠子宫内膜异位症的发病机制存在一定关系。复方莪术散可明显降低异位内膜组织中SDF-1、CXCR4表达，对子宫内膜异位症有一定治疗作用。