Effect of Compound Recipe Gengniankang on Senile Sexual Hormone and Expression of Estrogen Receptor in Bone of Climacteric Female Rats

摘要

Objective: To compare the therapeutic effect of Compound Recipe Gengniankang (GNK) with that of hormone replacement treatment (HRT) on climacteric female rats with osteoporosis, and to investigate the roles of estrogen and estrogen receptors in the mechanism of osteoporosis. Methods: Climacteric female rats with osteoporosis were chosen and divided into three groups (GNK group, HRT group and control group). Apoptosis of ovarian granulose cells was measured by terminal-deoxynucleotidyl transferae mediated nick end labeling (TUNED assay. Serum level of estrdiol (E_2), follicle stimulating hormone (FSH) , luteinizing hormone (LH) were determined by the method of radioimmunoassay (RIA). Reverse tran-scriptase polymerase chain reaction (RT-PCT) technology was used to evaluate the expression of estrogen receptor (ER) in bone. Bone mineral density (BMD) was measured by double energy X-ray absorption (DEXA). Results: In the climacteric rats, BMD, serum E_2, ER mRNA expression in bone decreased remarkably, and serum FSH, LH and apoptosis of ovarian granulose cells increased obviously. After treating with GNK, all the indexes were reversed except serum E_2. The increase of E_2 was not significant. Conclusion: GNK is effective on climacteric osteoporosis female rats. Its role is performed not by increasing serum E_2 but by enhancing ER in the bone and inhibiting apoptosis of ovarian granulose cells. GNK can deter further exhaustion of ovarian function.