龈沟液中基质金属蛋白酶8及基质金属蛋白酶组织抑制物1水平与牙周病的关系

摘要

目的 探讨龈沟液中基质金属蛋白酶8(matrix metalloproteinases-8,MMP-8)及金属蛋白酶组织抑制物1(tissue inhibitors of metalloproteinases,TIMP-1)的水平变化与牙周病的关系,以期为临床提供参考.方法 试验人群共48例,分为牙周健康组16例、慢性牙龈炎组18例及慢性牙周炎组14例,采用酶联免疫吸附测定法检测各组人群龈沟液中MMP-8和TIMP-1水平,并计算MMP-8与TIMP-1的比值.结果 牙周健康组、慢性牙龈炎组及慢性牙周炎组中MMP-8的平均质量浓度分别为:(0.62±0.09)、(0.68 ±0.02)及(0.78±0.05) mg/L,3组之间差异有统计学意义(P＜0.01),并且两两比较差异均有统计学意义(P＜0.05)；牙周健康组、慢性牙龈炎组及慢性牙周炎组龈沟液中MMP-8与TIMP-1的比值分别为:0.16 ±0.06、0.23±0.09及0.32 ±0.15,3组相比差异有统计学意义(P＜0.05).结论 龈沟液中MMP-8的质量浓度及MMP-8/TIMP-1比值的变化均可以作为判断牙周炎症水平有意义的临床指标.%Objective To investigate the relationship between the levels of matrix metalloproteinases(MMP)-8 and tissus inhibitors of metalloproteinases (TIMP)-1 in gingival crevicular fluid (GCF)and periodontal disease.Methods Fourteen patients with chronic periodontitis,18 cases with marginal gingivitis and 16 subjects with normal gingiva were selected in this study.The level of MMP-8 and TIMP-1 was detected by enzyme-linked immunosorbent assay (ELISA) and compared among the three groups.Results The average concentrations of MMP-8 of control group,chronic marginal gingivitis group and chronic periodontitis group were (0.62 ± 0.09),(0.68 ± 0.02) and (0.78 ± 0.05 ) mg/L,respectively.The difference was significant among the three groups (P ＜ 0.05 ).The average concentrations of TIMP-1 of the three groups were (3.55 ±0.93),(3.42 ± 1.49) and (2.88 ± 1.30) mg/L,respectively.There was no significant difference among the three groups ( P ＞ 0.05 ).The MMP-8/TIMP-1 ratios of the three groups were 0.16 ±0.06,0.23 ±0.09 and 0.32 ±0.15,respectively.The difference was significant (P ＜0.05).Conclusions The MMP-8 level and MMP-8/TIMP-1 ratio can be considered as two valuable clinical indicators for periodontal tissue destruction and inflammation degree.