涎腺多形性腺瘤中赖氨酰氧化酶表达的研究

摘要

Objective To investigate the expression of lysyl oxidase (LOX) in salivary pleomorphic adenoma.Methods The tissue samples were divided into two groups.Twenty samples of salivary gland pleomorphic adenoma tissues and twenty samples of normal parotid gland tissues adjacent to the pleomorphic adenoma were collected.The LOX gene mRNA expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Real-time PCR,and the alteration of expression was analysed.The LOX protein expression was then examined by western blotting.Results The salivary gland pleomorphic adenomatissue expressed more LOX gene mRNA and protein than normal salivary gland tissue.Conclusions LOX gene and protein may play a role in the tumorigenesis of salivary pleomorphic adenoma.%目的 检测涎腺多形性腺瘤(salivary pleomorphic adenoma,SPA)组织中赖氨酰氧化酶(lysyl oxidase,LOX)的表达,探讨LOX与SPA发生发展的关系.方法 取20例SPA组织标本,并配对20例SPA瘤旁涎腺组织,采用反转录聚合酶链式反应(RT-PCR)和实时荧光定量PCR方法检测LOX mRNA在组织中的的表达水平,并分析其表达变化,用蛋白质印迹法检测LOX的蛋白表达水平.结果 SPA组织中LOX mRNA表达水平明显高于瘤旁涎腺组织(12.81 ±0.92),SPA组织中LOX蛋白表达水平明显高于瘤旁涎腺组织.结论 LOX mRNA和蛋白在SPA瘤组织中均呈高表达,提示LOX的异常表达可能参与了SPA的发生、发展.