利用重测序的水稻染色体片段代换系定位控制稻米淀粉黏滞性谱QTL

摘要

The RVA profile is one of the important factors for rice cooking and eating quality evaluation, and it would be of great significance to identify new quantitative trail loci (QTLs) for RVA profile for improvement of rice quality. With Nipponbare as recipient and 9311 as donor, a population with 38 chromosome segment substitution lines (CSSLs) had been developed and subsequently genotyped by high-throughput resequencing strategy. The population with their parents similar in apparent amylose content was used for mapping QTLs of RVA properties including peak paste viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BDV), setback viscosity (SBV), consistence viscosity (CSV), peak time (PeT) and pasting temperature (PaT) in two years and two environments. The QTL analysis was carried out by using one-way ANOVA and Dunnett' s test, and stable QTLs were identified in both two years and two environments. 10 stable QTLs, qPKV2. 1 , qPKV5. 1 , qPKV7. 1 , qPKVS.1, qHPV5. 1, qHPV7. 1, qCPV5. 1, qCPV7. 1, qSBV2. 1 and qSBV7. 1 were identified in the chromosome 2, 5, 7 and 8, respectively, with contributions ranging from -31. 8% to 53. 2%. The QTLs in chromosome 2, 5 and 7 were found to have pleiotropy.%稻米RVA谱是评价稻米蒸煮与食用品质的重要指标之一,发掘新的控制稻米RVA谱QTL对稻米品质改良具有重要意义.利用以粳稻品种日本晴为受体、籼稻品种9311为供体并经高通量重测序的染色体片段代换系群体为材料,在两年两点的环境下对该群体中控制RVA谱特征值的QTL进行了定位分析.通过单因素方差分析和Dunnett多重比较,分析染色体片段代换系与受体亲本之间相关RVA谱特征值的差异,以两年两点都能检测到的显著差异位点作为稳定表达的QTL.共检测到10个稳定表达的QTL,包括控制峰值黏度的4个QTL qPKV2.1、qPKV5.1、qPKV7.1和qPKV8.1,控制热浆黏度的2个QTL qHPV5.1和qHPV7.1,控制冷胶黏度的2个QTL qCPV5.1和qCPV7.1及控制消减值的2个QTLqSBV2.1和qSBV7.1.在两年两点的检测中10个稳定表达QTL的贡献率介于-31.8％～53.2％,这10个QTL分布在第2、5、7和8染色体上,其中位于第2、5和7染色体上的位点存在一因多效性.