沈农系列水稻高产品种及其衍生系的遗传结构分析

摘要

Six high-yield varieties (Shennong 265 ,Shennong 606,Shennong 9741 ,Qianchonglang 1 ,Shennong 9903, Shennong 981 6)and 570 inbred lines were used as materials to analyze the utilization of seven cloned yield-related functional gene (DEP1 ,GS5 ,qSW5 ,Gn1a ,qGW8 ,GS3 and GW2 )and the contribution of indica blood in the japonica rice breeding of Liaoning Province.The results showed that allelic variations were detected from all of the functional gene loci except for GW2 .The loci of DEP1 ,qSW5 and qGW8 were widely used in japonica rice breeding by the breeders at Shenyang Agricultural University.In the 570 inbred lines,467 lines (82.1 3% of the lines)contained DEP1 ,550 lines (96.5 % of the lines)contained qSW5 ,and 35 1 lines (6 1 .6% of the lines)contained qGW8 .The functional alleles of GS5 ,Gn1a and GS3 were also present in a small number of lines,and the rates were 5 .44%, 0.70% and 1 .05 %,respectively.The results of the analysis with 32 indica-japonica specific markers showed that indica-japonica differentiation on these sites existed in 54.0% of the lines.Most of the indica genetic loci were located on chromosome 5 ,with a percentage of 20.8%.%以沈农265、沈农606、沈农9741、千重浪1号、沈农9903、沈农9816等6个高产品种及其衍生的570份品系为材料,通过对 DEP1、GS5、qSW5、Gn1a 、qGW8、GS3和GW2等7个功能基因位点的检测来分析这些已经克隆的控制产量性状的基因在辽宁水稻育种中的应用情况；同时,利用籼粳特异性的 InDel 标记分析籼稻血缘含量对北方高产优质粳稻育种的贡献.结果表明,除了 GW2外,其他6个功能基因位点均检测到等位基因的变异.576份材料中,DEP1、qSW5和 qGW8在沈农系列粳稻品种中被广泛利用.检测到467份材料含有 DEP1基因,占总数的81．9％；550份材料含有 qSW5基因,占96.5％；351份材料含有 qGW8,占61．6％；而 GS5、Gn1a 以及GS3在育种中被利用得较少,仅在少数品系中被利用,比例分别为5．4％、0．7％和1．1％.籼粳特异性标记分析表明,54．0％的参试材料在32个位点上有一定籼型血缘引入,且第5染色体上存在的籼型位点较多,占该染色体的20．8％.