VEGF-A，C，D 在人上皮性卵巢癌组织中的表达及临床意义

摘要

目的：检测血管内皮生长因子 A，C，D（VEGF-A ，VEGF-C ，VEGF-D）在人上皮性卵巢癌组织中的表达，探讨其表达水平与临床病理的关系。方法选择2003年1月—2008年12月在本院经手术治疗的上皮性卵巢肿瘤患者53例（恶性37例，交界性9例，良性7例），同期因子宫腺肌症行全子宫＋双附件切除的正常卵巢组织8例为对照组。采用免疫组化 SP 法检测两组标本中 VEGF-A，C，D 蛋白的表达水平，并分析其与与临床病理的关系。结果卵巢上皮性恶性肿瘤或交界性肿瘤中 VEGF-A，VEGF-C，VEGF-D 在肿瘤细胞胞浆或胞膜，间质中的血管内皮细胞及部分炎性细胞有表达。良性卵巢肿瘤及正常对照卵巢组织中三者均只表达于血管内皮细胞，但表达强度弱。低／中分化 VEGF-A 的阳性率明显高于高分化，Ⅲ—Ⅳ期 VEGF-A 的阳性率高于Ⅰ—Ⅱ期；低／中分化VEGF-C 阳性率高于高分化，Ⅲ—Ⅳ期 VEGF-C 阳性率高于Ⅰ—Ⅱ期，淋巴结转移的 VEGF-C 阳性率高于无淋巴结转移；低／中分化 VEGF-D 阳性率高于高分化，Ⅲ—Ⅳ期 VEGF-D 阳性率高于Ⅰ—Ⅱ期；差异均有统计学意义。结论 VEGF-A，C，D 在卵巢肿瘤的发生、发展中起重要作用，可能促进卵巢癌的浸润转移。%Objective To evaluate the expression of vascular endothelial growth factor (VEGF)-A,C,and D in human epithelial ovarian cancer,and to examine their relationship with clinicopathologic features. Methods The immunohistochemical method was used to determine the expression of VEGF-A,C,and D in 53 human epithelial ovarian neoplasm,of whom 37 cases were malignant,9 were borderline,and the other 7 were benign.These patients received operation in the our hospital from January 2003 to December 2008.Eight cases of normal ovarian tissues taken from patients with adenomyosis by panhystero-salpingo-oophorectomy were chosen as the control group. Results VEGF-A,VEGF-C, and VEGF-D expressed in cytoplasm or cytomembrane of malignant and borderline human epithelial ovarian cells,and in vascular endothelial and some inflammatory cells of stroma.But in benign and normal groups,they only expressed in vascular endothelial cells with a lesser extent.VEGF-A expression was stronger in poorly-moderately differentiated groups, and also stronger in stages Ⅲ and Ⅳ than in stages Ⅰ and Ⅱ.VEGF-C expression was stronger in poorly-moderately differentiated groups,stronger in stages Ⅲ and Ⅳ than stages Ⅰ and Ⅱ,and in lymph-node metastatic groups.VEGF-D expression was stronger in poorly-moderately differentiated groups,stronger in stages Ⅲ and Ⅳ than in stages Ⅰ and Ⅱ. Conclusion VEGF-A,C,and D proteins might play an important role in ovarian cancer development and in malignancy progression.They may be used as new target for therapy.