人脑多形性胶质瘤细胞系BT325对5种抗肿瘤药物敏感性的研究

摘要

Objective To examine the sensitivity of human glioblastoma multiforme cell line BT325 to 5 antineoplastic drugs, including cisplatin (DDP), teniposide (VM26), nimustine (ACNU), temozolomide (TMZ) and vincristine (VCR). Methods BT325 cells were incubated in DMEM with 10% or 20% fetal bovine serum (FBS) or without FBS respectively. The cell numbers were counted at 24 h, 48 h, 72 h, 96 h, 120 h, and 144 h, then platting and growth curve were drafted. Cell counting kite-8 was used to detect the influence of 5 drugs with different concentrations on human glioma cell line BT325. Results DDP and VM26 significantly suppressed BT325 cells(>75%) viability in a dose-dependent manner, while VCR inhibited BT325 cells (50%) growth without dose-effect relationship. In contrast, ACNU and TMZ were not effective on the viability of BT325 cells. Conclusion BT325 cells were very sensitive to chemotherapeutic drugs DDP amd VM26.%目的 检测人脑多形性胶质瘤细胞系BT325对顺铂(DDP)、替尼泊甙(VM26)、尼莫斯汀(ACNU)、替莫唑胺(TMZ)及长春新碱(VCR)5种临床常用抗肿瘤药物的敏感性,筛选脑胶质瘤化疗敏感药物.方法用含0％、10％及20％胎牛血清的DMEM培养基培养BT325胶质瘤细胞,分别在24 h、48 h、72 h、96 h、120h、144 h进行细胞计数,绘制细胞生长曲线,以确定细胞生长的最佳血清浓度；采用CCK-8法检测不同血浆峰浓度(PPC)5种药物对BT325胶质瘤细胞活性的影响,计算抗肿瘤药物的抑制率及IC50.结果细胞形态观察及细胞生长曲线显示含10％胎牛血清的DMEM培养基对BT325胶质瘤细胞生长较为适宜.细胞活性检测显示DDP在0.625×PPC(抑制率为51.8％)及以上浓度时可抑制BT325细胞生长,在1.25×PPC(抑制率为75.1％)及以上浓度时BT325细胞高度敏感,且有量效关系；VM26对BT325细胞抑制率均在79.6％以上,为高度敏感,剂量范围广且存在量效关系；VCR各浓度对BT325细胞抑制率为50％左右,但是与药物剂量无关；各浓度ACNU、TMZ对BT325细胞抑制率均在34％以下,无抑制作用.结论BT325胶质瘤细胞对DDP、VM26敏感,对VCR、ACNU、TMZ不敏感.